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Halt Protease and Phosphatase Inhibitor Cocktails FAQ


Answers to frequently asked questions (FAQs) about Halt Protease and Phosphatase Inhibitor Cocktails


 

What formats are available for the combined protease and phosphatase inhibitor cocktails?
The Halt Protease and Phosphatase Inhibitor Cocktails are offered as 100X concentrates in single-use or multiple-use formats. The metalloprotease inhibitor, EDTA, is provided in separate vials.

How much of the cocktail do I use for my sample?
The Halt Protease and Phosphatase Cocktail is supplied at a 100X concentrate and is used at 1X. For example, add 10µL of 100X Halt Cocktail to 1mL of sample. Some samples, however, contain high levels of proteases or phosphatase and might require a more concentrated treatment (i.e., 2-3X).

When do I need to add EDTA?
EDTA chelates the metal ions required for metalloprotease activity. Add EDTA if metalloproteases are likely to adversely affect target protein function; however, avoid using EDTA when the downstream application involves metal chelate affinity purification or the target protein requires metals for proper function.

Do you offer the components of the protease/phosphatase inhibitor cocktail individually?
Yes. Separate Halt Protease Inhibitor Cocktails and Halt Phosphatase Inhibitor Cocktails are available. In addition, some of the individual components of the Halt Protease and Phosphatase Inhibitor Cocktail are offered separately. All cocktail components and the corresponding product numbers for the components offered separately are listed below.

Component

Inhibitor Target

Product #

Aprotinin

Serine Proteases

78432

Bestatin

Aminopeptidase Proteases

78433

E-64

Cysteine Proteases

78434

Leupeptin

Seine and Cysteine Protease

78435

Sodium Fluoride

Serine/Threonine and Acidic Phosphatases

N/A

Sodium Orthovanadate

Tyrosine and Alkaline Phosphatases

N/A

b-glycerophosphate

Serine/Threonine Phosphatase

N/A

Sodium Pyrophosphate

Serine/Threonine Phosphatase

N/A

EDTA

Metalloprotease

17892

What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?
Although we reveal the identity of the inhibitors in the combined cocktail, their concentrations are proprietary.

Can I store the 1X solution and if so, for how long?
The stability of the 1X solution is undetermined. For best results, prepare solutions just before use. The 100X concentrate is stable for at least one year stored at 4°C.

 

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