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Epitope Tag Product Selection Guide

Choose an appropriate fusion tag-based product for purification or immunoprecipitation.

Anti-epitope tag magnetic beadsProtein biology research is increasingly mediated and facilitated at one step or another by the use of recombinant proteins. Whether produced using in vivo or in vitro expression systems, recombinant proteins are powerful tools for exploring and measuring protein interaction and cellular functions. In part, this is because they can be designed as partial or specific alterations of native protein sequences. More importantly, recombinant proteins can be engineered to contain sequences that specify particular affinity tags to facilitate purification and/or detection by a variety of methods.

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Protein Methods Library Home


Overview of Protein Expression

Overview of Affinity Purification

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Fusion Protein Purification Resins, Columns and Kits

Pierce Magnetic Beads

Description

The four most popular fusion tags for recombinant protein expression:

  • His Tag – consists of a string of six to nine histidine residues; primarily used for purification via immobilized metal affinity chromatography (IMAC)
  • GST Tag – consists of glutathione S-transferase (GST), a complete 211 amino acid protein (26kDa); primarily used for purification via glutathione agarose resin
  • HA Tag – consists of a peptide (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein; immobilized anti-HA antibodies are used to immunoprecipitate HA-tagged recombinant proteins
  • c-Myc Tag – consists of a peptide (EQKLISEEDL) derived from the human c-myc oncogene (p62 c-myc); immobilized anti-c-Myc antibodies are used to immunoprecipitate c-Myc-tagged recombinant proteins

The former two tags (His and GST) are not properly called epitope tags, because they are not usually purified or detected via specific antibodies. By contrast, HA and c-Myc tags are properly called epitope tags, because their only means of purification or detection is via specific antibodies. Epitope tags are seldom used for bulk purification but are most often used for immunoprecipitation or co-immunoprecipitation (IP, co-IP). Nevertheless, all four of these tag systems can be used for either purification or pull-down applications.

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His-tagged Proteins

GST-tagged Proteins

 

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Immunoprecipitation and Pull-Down Kits

Quick-link Table of Products

Links to different types of epitope-tag and affinity-tag products. Numbers under each tag are representative part numbers from the linked product pages. This table features links to specific detection antibodies and affinity supports for purification, immunoprecipitation or pull-down assays. Also available are expression vectors for in vitro translation (IVT) systems and coated microplates for assay development.
 Product Type HA c-Myc His GST
Peptides 26184 20170 20237
Antibodies 26183 MA121316 MA121315 MA4004
Agarose Resins 26181 20168 89964 16100
Magnetic Beads 88836 88842 88831 88821
IP and Co-IP Kits – Agarose 26180 23620 21277 21516
IP and Co-IP Kits – Magnetic 88838 88844
qIP Protein Interaction Kits – Agarose 82032 82033
qIP Protein Interaction Kits – Magnetic 82035 82036
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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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