Update profile
    Change password
    Sign out

Gel Filtration Chromatography Products Selection Guide

Choose the appropriate size exclusion chromatography resin and column size.

Gel filtration chromatography is often used to perform desalting and buffer exchange with the aid of various size exclusion chromatography resins. Desalting involves the chromatographic separation of macromolecules from smaller molecules such as buffer components, salts and unreacted labeling reagents. Buffer exchange is used to switch a protein or molecule into a more appropriate buffer for applications such as electrophoresis, ion exchange, affinity chromatography or storage.

In both desalting and buffer exchange, the macromolecular components are recovered in the same buffer used to equilibrate the resin within the gel filtration column. If water is used for equilibration, the components will be desalted. If another buffer is used, buffer exchange will result. Buffer exchange may be performed with each of the desalting products listed below after pre-equilibration with the desired buffer.

Page Contents:

Learn more...

Protein Methods Library Home


Overview of Size Exclusion Chromatography for Buffer Exchange

Related literature...
Zeba Desalting Handbook
Zeba Desalting Handbook

Zeba-resin Gel Filtration Chromatography Products

Thermo Scientific Zeba Desalting Products contain a proprietary high-performance size exclusion resin that offers exceptional desalting and protein-recovery characteristics compared to other commercially available resins. Even very dilute (25µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules and good recovery of proteins and other macromolecules.

The Zeba Spin Desalting Columns (available in five column sizes) and two MWCOs (7K and 40K) provide an easy-to-use format that dramatically improves results over standard drip-column methodologies. This eliminates the need to wait for samples to emerge by gravity flow and monitor fractions for protein recovery. Zeba Desalting Columns require no chromatography instrumentation, cumbersome column preparation or equilibration and they can process multiple samples in approx. 8 minutes.

Zeba Desalting Resin also comes pre-packed in 96-well filter/centrifuge plates and in two sizes of Pierce Chromatography Cartridges.

Zeba Desalting Resin Products and recommended sample volumes.
7K MWCO 40K MWCO Resin Bed Sample Size
micro spin column micro spin column 0.075mL 2 to 12µL
0.5mL spin column 0.5mL spin column 0.5mL 30 to 130µL
2mL spin column 2mL spin column 2mL 200 to 700µL
5mL spin column 5mL spin column 5mL 600 to 2000µL
10mL spin column 10mL spin column 10mL 1500 to 4000µL
96-well spin plate 96-well spin plate 0.55mL 20 to 100µL
1mL cartridge   1mL 50 to 250µL
5mL cartridge   5mL 100 to 1500µL
Note: Sample size ranges given here are for the 7K MWCO products. Ranges for the 40K MWCO products are slightly different; see the product page for specific values.

 

Zeba-resin Gel Filtration  Chromatography Spin Desalting Columns

View products...

Zeba Spin Desalting Columns, Plates, Cartridges (7K MWCO)

Zeba Spin Desalting Columns
and Plates (40K MWCO)

Zeba-resin Gel Filtration Chromatography Spin Plates and Cartridges

Related products...

Empty Gel Filtration Chromatography Spin Columns

Polyacrylamide Size Exclusion Chromatography Columns and Spin Columns

Thermo Scientific Polyacrylamide Desalting Columns are pre-packed with polyacrylamide size exclusion resin. The 6K MWCO columns have a wet bead diameter of 90 to 180µm. The 1.8K MWCO columns have a wet bead diameter of 45 to 90µm. These columns are ideal for separating low MW compounds with a fractionation range of 0.1 to 1.8K. The polyacrylamide size-exclusion resin is not subject to enzymatic degradation and will not serve as a nutrient for microbial growth. The resin is very hydrophilic, so hydrophobic binding to sample molecules is minimized. Polyacrylamide resins also eliminate sample contamination by low MW sugars which can occur when using crosslinked polysaccharide supports. In addition, oxidizing agents can be removed without destroying the support. This resin is susceptible to hydrolysis of amide groups under extreme pH conditions, so an operating pH of 2 to 10 is recommended at room temperature. The polyacrylamide resin can also be autoclaved at pH 5.5 to 6.5 for 30 minutes at 120°C.

Polyacrylamide Desalting Columns and recommended sample volumes.
Column Size Resin Bed Sample Size
0.7mL spin column 0.7mL 30 to 120µL
5mL gravity column 5mL up to 1.25mL
10mL gravity column 10mL up to 2.5mL
Note: The optimal sample size for this matrix is 1/10 the column resin bed. However, sample volumes up to 1/4 of the column volume bed may be used if 100% desalting is not required.

 

Polyacrylamide-resin Gel Filtration Chromatography Columns

View products...

Polyacrylamide Gel Filtration Chromatography Columns

Dextran-resin Gel Filtration Chromatography Columns

Thermo Scientific Dextran Desalting Columns (5K MWCO) are pre-packed with crosslinked dextran size exclusion resin. The wet bead diameter is 50 to 150µm. The resin has good rigidity for easy handling and excellent flow properties. The Dextran resin is stable in water, salt solutions, organic solvents and alkaline or weakly acidic solutions. It is heat-stable and can be autoclaved dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties.

Dextran Desalting Columns and recommended sample volumes.
Column Size Resin Bed Sample Size
5mL gravity column 5mL up to 1.25mL
10mL gravity column 10mL up to 2.5mL
Note: The optimal sample size for this matrix is 1/10 the column resin bed. However, sample volumes up to 1/4 of the column volume bed may be used if 100% desalting is not required.

 

Dextran-resin Gel Filtration Chromatography Columns

View products...

Dextran Gel Filtration Chromatography Columns

Browse all dialysis and desalting products


Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747