Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents contain two or more reactive ends that are capable of attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules.
Only a small number of reactive chemistries are used as the basis for synthesizing the reagents that are most popular for protein conjugation techniques. However, these five or six types of reactive groups can be combined in a variety of ways and attached to molecular backbones of various lengths and chemical properties to create many specific crosslinking reagents.
Choosing among the commercially available options can seem overwhelming until one considers the particular application needs and categorizes reagents by their general features. The following two tables provide a comprehensive list of Thermo Scientific Pierce Crosslinking Reagents classified as succinctly as possible by their most important features.
Overview of Crosslinking
Chemistry of Crosslinking
Crosslinker Selection Guide
(Interactive version of this page)