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Reducing Agents for Protein Disulfides


Purified powders, convenient solutions and solid-phase resins of disulfide reducing agents, including DTT, BME and TCEP, for stabilization of free sulfhydryls (cysteines) and reduction of disulfide bonds in peptides and proteins.


Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines). 

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds. 

Stable, 0.5M solution of TCEP reducing agent for protein disulfide bonds; odor-free; ideal as 10X stock to make reducing SDS-PAGE sample buffers. 

Pure, crystalline cysteine hydrochloride (Cys-HCl) CAS 52-89-1; use to make sulfhydryl assay standards or as an additive in protein refolding experiments. 

Pure, crystalline dithiothreitol (DTT), CAS 3483-12-3; popular protein disulfide reducing agent for sample loading buffers; convenient package sizes. 

Beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized to enable efficient reduction of peptide or protein disulfide bonds. 

Beaded resin on which a thiol-based reducing agent is immobilized to enable solid-phase reduction of peptide or protein disulfide bonds; reusable. 

Pure, crystalline Tris (2-carboxyethyl) phosphine hydrochloride, CAS 5961-85-3 (TCEP), a thiol-free reductant for protein and peptide disulfide bonds. 

Popular protein disulfide reducing agent, prepared as thoroughly tested, highly characterized and fully documented batches of reagent. 

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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