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Pierce Concentrated Buffers


10X and 20X concentrated solutions of frequently used sample, dialysis, electrophoresis, transfer and storage buffers for protein research techniques; simply dilute to 1X with water and they're ready to use.


Prepare sodium borate buffer solutions for protein modification procedures requiring amine-free buffer at alkaline pH with this space-saving stock solution. 

Prepare Na- and K-phosphate buffered saline (D-PBS) wash and blocking buffers for ELISA, Western and other immunoassays with this space-saving stock solution. 

Prepare Na- and K-phosphate buffered saline (D-PBS) diluents for ELISA, Western blotting and other immunoassays with this space-saving stock solution. 

Prepare PBS-Tween (PBS-T) wash buffers and blocking buffers for ELISA and Western with this space-saving stock solution. 

Prepare PBS buffers for crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer with this space-saving stock solution. 

Cast agarose gels and prepare Tris-Acetate EDTA (pH 8.3) running buffer for nucleic acid electrophoresis with this space-saving stock solution. 

Cast agarose gels or prepare Tris-borate-EDTA (pH 8.3) running buffer for agarose and polyacrylamide gel electrophoresis with this space-saving stock solution. 

Prepare TBS-Tween (TBS-T) wash buffers or blocking buffers for ELISA, Western blotting and other immunoassays with this space-saving stock solution. 

Prepare Tris-NaCl buffer (TBS) used as a wash buffer and antibody diluent for ELISA, Western blotting and other immunoassays with this space-saving stock solution. 

Quickly prepare standard Tris-glycine (pH 8.5) transfer buffer for Western blot membrane with this space-saving stock solution. 

Quickly prepare Tris-glycine-SDS (pH 8.3) running buffer for SDS-PAGE (protein polyacrylamide gel electrophoresis) with this space-saving stock solution. 

Prepare Tris-HEPES-SDS running buffer required for gel electrophoresis (SDS-PAGE) with Precise Precast Gels with this space-saving stock solution. 

Thermo Scientific Pierce 10X Western Blot Transfer Buffer, Methanol-free is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) Western blotting transfer appratus. 

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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