Simplify the selection of a blocker for Western blot and ELISA applications.
Thermo Scientific StartingBlock Blocking Buffers are PBS and TBS formulations of a proprietary protein that provides exceptional performance and broad compatibility with membrane (Western blot) and 96-well plate (ELISA) assays.
Although no blocking buffer is ideal for every experimental situation, you are likely to obtain excellent results from the start with StartingBlock Blocking Buffers. This is because the blocking agent performs well with an extremely wide range of antibodies and assay systems. Our scientists have yet to encounter an antibody combination or other protein probing system in which StartingBlock Blocking Buffer is not compatible.
Compatible with many detection systems – Western blot, ELISA and IHC with antibody or avidin/biotin probes (blocker is serum and biotin-free)
Short blocking times – less than 15 minutes for nitrocellulose or PVDF membranes, almost instantaneous for polystyrene microplate wells
Strip and reprobe without reblocking – blots stay blocked even after stripping with Thermo Scientific Restore Stripping Buffer (Part No. 21059)
High signal:noise ratio – biotin- and serum protein-free formulation contributes to acheiving signal-to-noise ratios from 10:1 to 20:1
Convenient formats – available ready-to-use 1X formulations in PBS and TBS, with and without Tween*-20 detergent
Thermo Scientific StartingBlock Blocking Buffer performance after stripping and reprobing. Nitrocellulose and PVDF membranes that had been blocked with StartingBlock Blocking Buffer and used to detect a different primary target were then stripped with Thermo Scientific Restore Western Blot Stripping Buffer (Part No. 21059) and probed for the transferrin receptor (CD71). Detection was with SuperSignal West Dura Chemiluminescent Substrate (Part No. 34075) and exposed to film for 30 minutes and then again 24 hours. Very little background occurs with either membrane or exposure time, indicating exceptional blocking performance.
Alegria-Schaffer, A., et al. (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.