DyLight 405
Fluorescent dye properties, example data, product guide and references.
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Thermo Scientific DyLight 405 provides exceptional performance for a wide array of fluorescence-base applications at a lower cost than other 405-range dyes. DyLight 405 has a narrow excitation wavelength that makes the dye ideal for multi-color applications and is available as a reactive labeling agent and a conjugate of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.
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Fluorescent Probes
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DyLight Fluor Product Guide
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DyLight 405 Properties and Applications
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| Thermo Scientific DyLight 405 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (blue) spectra are normalized to the same height in this graph. |
Properties of Thermo Scientific DyLight 405.
| Parameter |
Value |
| Excitation / emission maxima |
400nm / 420nm |
| Emission color |
Blue |
| Molar extinction coefficient (ε) |
30,000 M-1 cm-1 |
| Correction factor (A260/A400) † |
0.564 |
| Molecular weight |
NHS ester: 793g/mol
Maleimide: 818g/mol
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| Spectrally similar dyes |
Alexa Fluor* 405, Cascade* blue, AMCA |
| † Correction factor can be used to estimate protein labeling efficiency. |
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Compare spectra and properties among all DyLight Fluors
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Fluorescent Probes: Description, Methods and Applications |
DyLight 405 Products
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Related products:
DyLight Specialty Dyes
DyLight Fluor Product Guide |
DyLight 405 References
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- Currier, T. A. et al. (2010) The Effects of Embryonic Knockdown of the Candidate Dyslexia Susceptibility Gene Homologue Dyx1c1 on the Distribution of Gabaergic Neurons in the Cerebral Cortex. Neuroscience
- Dimitrov, E. and Usdin, T. B. (2010) Tuberoinfundibular peptide of 39 residues modulates the mouse hypothalamic pituitary adrenal axis via paraventricular glutamatergic neurons. The Journal of Comparative Neurology.
- McNerny, D. Q. et al. (2009) RGD Dendron Bodies; Synthetic Avidity Agents with Defined and Potentially Interchangeable Effector Sites That Can Substitute for Antibodies. Bioconjugate chemistry 20, 1853-9.
- Rotzius, P. et al. (2010) Distinct Infiltration of Neutrophils in Lesion Shoulders in ApoE-/- Mice. Am. J. Pathol. 177, 493-500.
- Speidl, W. S. et al. (2010) The complement component C5a is present in human coronary lesions in vivo and induces the expression of MMP-1 and MMP-9 in human macrophages in vitro. FASEB J fj.
- Zhang, X. and Wong, S. M. (2009) Hibiscus chlorotic ringspot virus upregulates plant sulfite oxidase transcripts and increases sulfate levels in kenaf (Hibiscus cannabinus L.). J. Gen. Virol. 90, 3042-50.
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Fluorescent Labeling and Detection Products Guide
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