Microplate sandwich ELISA kits for human interleukin 1 alpha (IL-1a).
Assay IL-1α (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-1 alpha ELISA Kits.
These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Highlights:
Target: natural and recombinant human interleukin-1 alpha (IL-1α)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA.
Human IL-1α ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-1α ELISA Kit (Part No. EH2IL1A), with error bars based on five replicates for each interleukin-1 alpha standard concentration.
