Sandwich ELISA for human interleukin-8 (IL-8) with ECL substrate.
Assay IL-8 (human interleukin-8) levels in human serum, plasma and culture supernatant samples by enhanced chemiluminescence (ECL) with our Thermo Scientific Human IL-8 Chemiluminescent ELISA Kit.
This human IL-8 ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate chemiluminescent measurement of interleukin-8 (IL-8) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP and Thermo Scientific SuperSignal Chemiluminescent Substrate solutions. The complete kit contains pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant human interleukin-8 (IL-8)
Format: chemiluminescent ELISA (SuperSignal Substrate) in black 96-well strip plate
Measurement: luminometer detection of ECL light emission (max. Em = 425nm)
Human IL-8 ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-8 Chemiluminescent ELISA Kit (Part No. 84774), with error bars based on twelve replicates for each interleukin-8 standard concentration.