Assay MBL (human oligomerized mannan-binding lectin, also called mannose-binding protein) in human serum or heparin plasma with our Thermo Scientific Human MBL Oligomer ELISA Kit.
This ELISA kit is for the determination of oligomerized mannan binding lectin in human serum or heparin plasma. The assay is an ELISA performed in microplate wells coated with a monoclonal antibody against the MBL carbohydrate-binding domain. Bound MBL is detected with the same antibody that has been labeled with biotin, followed by development with horseradish peroxidase (HRP)-conjugated streptavidin and incubation with a chromogenic substrate. Comparison of the assay results with molecular size chromatography of MBL immunoreactivity in individual human serum samples suggests that the monoclonal antibody used is selective for MBL oligomers when used as both capture and detection antibody. The assay is a four-step procedure.
- Target: human mannan-binding lectin (MBL), also called mannose-binding lectin or protein
- Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
- Measurement: absorbance at 450nm minus absorbance at 550nm
- Assay Range: 0.4-40ng/mL (= at least 4ng/mL MBL in original, undiluted samples)
- Sensitivity: < 0.1ng/mL
- Precision: undetermined
- Sample Types: serum, plasma (heparin)
- Total Assay Time: 2.5 hours
- Sample Size: 100µL of 10-fold diluted sample per well
- Specificity: Western blotting of plasma or plasma fractions has not identified bands reacting with the antibody other than those attributable to MBL. The absence of cross-reaction with other plasma components is supported by the fact that readings indistinguishable from zero are obtained from some MBL-deficient but otherwise normal donors.
Complete kit contains pre-coated antibody-plate, detection antibodies, buffer, diluent, standard, and substrate.
Mannan-binding lectin (MBL; also called mannose binding lectin or protein) is a multimeric carbohydrate-binding protein produced in the liver and secreted into the blood, where it constitutes an important element in innate immune defense against invading microorganisms. Its normally oligomerized forms are associated with specific serine proproteases (the MASPs) which are activated when MBL binds to microbial carbohydrate surfaces and in turn activate complement via the MBL or lectin pathway. The determination of MBL concentrations in serum may be useful for the elucidation of suspected immune defects and as a prognostic indicator alerting to the need for heightened therapeutic or prophylactic measures in immunosuppressed patients, including patients receiving cancer chemotherapy, and patients with cystic fibrosis, SLE or rheumatoid arthritis.