DMP (Dimethyl pimelimidate•2 HCl)

Thermo Scientific Pierce DMP is dimethyl pimelimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of a 7-atom spacer arm.

Highlights

• Water-soluble
• Rapid reaction with amines at alkaline pH values (pH 8-10)
• Amidine bond retains net charge character of protein
• Reversible at high pH values
• Reactive groups: imidoester (homobifunctional)
• Reactive toward: amino groups
• Reversible amidine bond retains net charge character of proteins

Product Details:

References

1. Bauer P.I., et al. (1990). Biochem. J. 270(1), 17-26
2. O’Keeffe, E.T., et al. (1980). Biochemistry 19(22), 4962-4966.
3. Schneider, C., Newman, R.A., Sutherland, D.R., Asser, U. and Greaves, M.F. (1982).
   A one-step purification of membrane proteins using a high efficiency immunomatrix.
   J. Biol. Chem. 257(18), 10766-10769.
4. Sinha, S.K. and Brew, K. (1981). J. Biol. Chem. 256(9), 4193-4204.