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Endogen offers a broad range of recombinant cytokines, chemokines, growth factors and active kinases for bovine, canine, equine, feline, human, mouse, porcine, primate, rat and Rhesus monkey. All of Endogen’s proteins are fully validated and QC-tested (N-terminal sequence, bioassay and SDS-PAGE analysis) to ensure high biological activity and accurate, reproducible results. Applications include cell culture maintenance, bioassays, in vivo research and standards for Western blot and ELISA. Whenever possible, new Endogen recombinant proteins will be expressed in a yeast system, Pichia pastoris, which generates proteins that are secreted, properly folded, and glycosylated in a manner similar to mammalian systems. Most proteins are provided carrier-free and low endotoxin. A lot-specific Certificate of Analysis is provided with each protein.
Species: Feline
Analyte: TNF alpha
Type: Recombinant protein
Activity: The biological activity of recombinant feline TNF a was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D.
Format: Lyophilized, preservative and carrier free
Expression: Mature, soluble form of feline TNF a. GenBank Accession # P19101,
amino acids 77 - 233.
Protein Source: Pichia pastoris
Molecule Weight: 17.3 kDa
Purity: >95%
Homology:
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Bovine TNFα
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Canine TNFα
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Equine TNFα
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Feline TNFα
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Human TNFα
|
Mouse TNFα
|
Ovine TNFα
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Porcine TNFα
|
Rat TNFα
|
Rhesus macaque TNFα
|
|
|
100
|
79
|
79
|
80.3
|
80.9
|
75
|
92.4
|
83.3
|
74.4
|
80.9
|
Bovine
TNF α
|
|
-
|
100
|
89.2
|
95.5
|
93.6
|
79.5
|
79.6
|
86.5
|
78.8
|
94.3
|
Canine
TNFα
|
|
-
|
-
|
100
|
89.2
|
89.2
|
80.1
|
77.7
|
84
|
78.8
|
89.2
|
Equine
TNFα
|
|
-
|
-
|
-
|
100
|
93
|
80.1
|
80.9
|
86.5
|
79.5
|
93.6
|
Feline
TNFα
|
|
-
|
-
|
-
|
-
|
100
|
79.5
|
80.9
|
87.2
|
78.8
|
98.1
|
Human
TNFα
|
|
-
|
-
|
-
|
-
|
-
|
100
|
73.7
|
76.9
|
94.9
|
79.5
|
Mouse
TNFα
|
|
-
|
-
|
-
|
-
|
-
|
-
|
100
|
83.3
|
74.4
|
80.9
|
Ovine
TNFα
|
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
100
|
76.3
|
87.2
|
Porcine
TNFα
|
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
100
|
78.8
|
Rat
TNFα
|
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
-
|
100
|
Rhesus
macaque
TNFα
|
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