Thermo Scientific Precise Protein Gels are precast polyacrylamide gels for protein electrophoresis (PAGE) that use Tris-HEPES running buffer, have long shelf lives and provide compatibility with different commercial gel tanks.
Precise Gels are cast in a durable plastic cassette using a neutral-pH buffer that prevents polyacrylamide breakdown and results in a long shelf life. High-resolution staining and transfer of proteins is accomplished quickly on these 1mm thick mini gels. Gels are individually packaged in an easy-to-open plastic pouch and are ready to run with no comb or tape to remove. Following electrophoresis, the cassettes are easy to dismantle (no special tools required) without damaging the gel. Precise Gels are available in gradient (4 to 20% and 8 to 16%) and fixed (8%, 10% and 12%) concentrations and in 10-well, 12-well and 15-well formats.
High-performance – unique running buffer produces excellent separation and high-resolution protein bands
Long shelf life – up to 12-month stability ensures consistent performance for the duration of each package of gels
Fast – 45-minute run-time provides results quickly; transfers efficiently to nitrocellulose and PVDF membranes in just 90 minutes; stains quickly coomassie and silver stains
Large-volume wells – innovative well design accommodates twice the sample volume of typical mini-gel cassette wells (10-well=50µL, 12-well=30µL; 15-well=25µL)
Compatible – cassettes fit many popular mini-gel tanks, and gels can be loaded using standard Laemmli sample loading buffers
Several convenient styles – choose from five gel densities and three well formats
Chart of resolution and migration profiles for Thermo Scientific Precise Protein Gels. Values in each lane are protein sizes in kilodaltons (kDa) for the tested protein molecular weight marker.
Hoefer Tall Mighty Small* (SE 280), Mighty Small* II (SE 260/ SE 250), Mini VE System (SE 300)
C.B.S. Scientific MGV 302/402
Daiichi Mini 2-Gel and 6-Gel
GradiGel Mini 4-Cell
SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is used to determine the approximate molecular weights of proteins with reference to the mobility of standard proteins on the same gel. Gradient gels provide the appropriate gel pore size, making molecular weight estimation more accurate by sharpening stained protein bands.
The most popular buffer system for SDS-PAGE is the Laemmli system [Nature 227, 680-686 (1970)]. This system, however, is limited by extended running times and gel instability from hydrolysis of polyacrylamide to acrylic acid in alkaline conditions. This chemical change alters gel conductivity, causing a constant change in protein migration patterns with time.
Polyacrylamide hydrolysis does not occur at neutral pH, and Thermo Scientific Precise Protein Gels are cast at pH 7, yielding a long shelf life and assured reproducibility of protein migration patterns. The extra stability of Precise Protein Gels is combined with a new Tris-HEPES running buffer to give speed and excellent resolution with the same size ranges as the Laemmli system. The buffer can be made according to the recipe provided in the instruction booklet or purchased premixed.