Mouse IFN alpha ELISA Kit

Assay IFN-α (mouse interferon alpha) levels in mouse cell and tissue culture media (supernatants) with this VeriKine* Mouse IFN alpha ELISA Kit (for Supernatant).

This mouse IFN alpha ELISA kit uses pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon alpha (IFN-α) in samples at 100μL per well. The IFN-α kit is validated for use with mouse cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kit contains pre-coated 96-well plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: mouse interferon alpha (mouse IFN-α, IFN-alpha)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
• Measurement: absorbance at 450nm
• Assay Range: 12.5 to 400pg/mL
• Sensitivity: undetermined
• Precision: intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
• Sample Types: cell culture supernatant, tissue culture media
• Total Assay Time: 27.25 hours (overnight)
• Sample Size: 100μL
• Specificity: no significant cross-reaction with mouse IFN-β, IFN-γ; human or rat IFN-α.

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, buffers, diluents, standards, substrate, and stop solution.

Details:

Mouse IFN-α ELISA standard curve. An example standard curve for the VeriKine* Mouse IFN-α ELISA Kit for Supernatants (Part No. 421201), with error bars based on three replicates for each interferon alpha standard concentration.

References:

• IFN alpha ELISA Kit literature references

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search IFN alpha Antibodies (www.pierce-antibodies.com) – matched pair links coming soon