This bovine IL-1 beta ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Highlights:
Target: natural and recombinant bovine interleukin-1 beta (IL-1β)
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 31 to 2000pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3.5 hours
Sample Size: 100μL
Specificity: no significant cross-reaction with recombinant bovine IL-2, IL-4, IL-6, IL-8, IFNγ, or TNFα.
Includes:
The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
Details:
Bovine IL-1 beta ELISA standard curve. An example standard curve for the Thermo Scientific Bovine IL-1 beta ELISA Reagent Kit (Part No. ESS0027), with error bars based on six replicates for each interleukin-1 beta standard concentration.
