Thermo Scientific DyLight 350 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 350 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.
DyLight 350 has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Alexa Fluor 350 and AMCA dyes in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.
Highlights:
- High performance – DyLight 350 shows brighter fluorescence than Alexa Fluor 350 and AMCA
- Specific – maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
- Efficient labeling methods – well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
- Optimized antibody labeling procedure –complete protocol for IgG reduction and labeling and calculating the labeling efficiency
Applications:
- Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
- Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
Product Details:
DyLight 350 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. (Learn more about maleimide chemistry.)
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