Improved dialysis units for samples containing proteins larger than 10kDa.
Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes (10K MWCO) provide maximum convenience, flexibility and performance for sample dialysis.
These second generation (G2) Slide-A-Lyzer Dialysis Cassettes are free-standing, self-floating and pipette-loadable units for dialysis. The single-use, disposable cassettes are available in five different sizes to accommodate samples between 0.25mL and 70mL. They offer maximum efficiency, convenience and sample protection in one package. Sample loading and removal are easily accomplished by using a serological pipette or hypodermic needle (optional) attached to a syringe. The built-in air chamber provides sample buoyancy and vertical orientation of the cassette during dialysis.
Clean and sterile versions – standard clean-room and gamma irradiated packages available
Easy-loading – pipette-accessible for easy sample loading and retrieval
Self-floating – integrated air chambers eliminate the need for float buoys
Sturdy construction – ensures the highest possible sample integrity and protection
Superior design – thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery
Multiple sizes – five cassette capacities to optimally match 0.25 to 70mL sample volumes
Versatile – ideal for removing low molecular-weight contaminants, performing buffer exchange and desalting
10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30µm)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace
The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be loaded with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports.
The 10K molecular-weight cutoff (MWCO) cassettes use a low-binding regenerated cellulose that is rated to completely retain proteins and other macromolecules that are larger than 10,000 daltons while allowing removal of buffer salts and small contaminants. Membranes and cassettes are manufactured using clean-room conditions to ensure that are contaminant-free. The Slide-A-Lyzer G2 Dialysis cassettes are available in five precise membrane MWCOs (2K, 3.5K, 7K, 10K and 20K) for dialyzing sample volumes up to 70mL.
Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications in which sterile conditions are required to minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 15-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth.
Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassettes make sample loading and recovery easy. The Slide-A-Lyzer G2 Dialysis Cassettes allow samples to be directly injected with a syringe or with a pipette through the unique sample port. The Slide-A-Lyzer Cassette design allows air to be removed from inside the cassette to maximize the surface area-to-volume ratio, providing better dialysis performance than dialysis tubing and tube-based systems. The sample is easily recovered through either the pipette or syringe sample ports. Watch the Slide-A-Lyzer G2 Dialysis Cassette Demo video.