Automated Magnetic Separations for Proteomics
Accelerate proteomics workflow throughput (HTS) using magnetic separations.
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 Speed and sample purity are major concerns for proteomics researchers. The amount of work required for proteomic analysis necessitates higher sample- and data-processing throughput. Magnetic particles comprise a convenient solid support for a variety of assays and procedures based on affinity purification. Paramagnetic beads are especially well suited for automated procedures because instrumentation exists to easily mix, incubate and separate the particles in 96-well plates without columns or centrifugation. Such ease of manipulation enables rapid processing of many samples and seemless integration and compatibility with entire workflows.
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Overview of Affinity Purification
Overview of Immunoprecipitation
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Automation-validated Magnetic Beads
Thermo Scientific Pierce Magnetic Beads are specially designed and validated for use with automated magnetic particle processors. We offer glutathione and titanium dioxide magnetic beads for reliable and quick sample purification of the respective affinity targets. The high-performance, iron oxide, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher Flex Magnetic Particle Processor.
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Affinity Purification
Immunoprecipitation Kits |
Types and applications of Pierce Magnetic Beads.
| Magnetic Bead Ligand |
Affinity Target |
Primary Applications |
Compatible Analysis Methods |
| Anti-HA |
HA-tagged fusion proteins |
HA fusion protein purification from lysates |
SDS-PAGE
Western blot
Mass spectrometry |
| Ni-NTA |
His-tagged fusion proteins |
His fusion protein purification from lysates |
SDS-PAGE
Western blot
Mass spectrometry |
| Glutathione |
Glutathione S-transferase (GST) fusion proteins |
GST fusion protein purification from lysates |
SDS-PAGE
Western blot
Mass spectrometry |
Protein A/G
Protein A
Protein G
Protein L |
Antibodies (IgG) |
Antibody purification, immunoprecipitation (IP) and co-IP |
SDS-PAGE
Western blot
Mass spectrometry |
| Streptavidin |
Biotin |
Affinity purification involving biotinylated molecules |
SDS-PAGE
Western blot
Mass spectrometry |
| Titanium Dioxide |
Phosphopeptides containing phosph- Ser, Tyr or Thr |
Isolate phosphopeptides from complex biological samples |
Mass spectrometry |
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Thermo Scientific Automated Solutions
Together with Thermo Scientific Instruments for magnetic particle processing and mass spectrometry, Thermo Scientific Pierce Magnetic Beads and cell lysis reagents provide a complete solution for proteomics research.
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Broad Instrument Compatibility
Our Pierce Magnetic Beads are also compatible with other instrumentation designed for magnetic particle processing (see table below). The beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand. (Thermo Scientific MagnaBind Beads are suitable for primarily manual purification methods and are available in several additional formats, including streptavidin and amine-derivatized forms.)
Automated robotic magnetic particle processors that are compatible with Thermo Scientific Pierce Magnetic Beads.
| Instrument |
Manufacturer |
| KingFisher* |
Thermo Fisher Scientific |
| Biomek* FX |
Beckman Instruments |
| Gene-Pure |
Bioer |
| Chemagic |
Chemagen |
| iPrep* |
Invitrogen |
| DNATrak*, Evolution*, MultiPROBE* II |
PerkinElmer |
| Maxwell* 16 |
Promega |
| BioCube* |
Protedyne |
| Biorobot*, QIAsymphony*, QIAcube* |
Qiagen |
| Magna Pure* LC |
Roche |
| Genesis FE500*, Freedom EVO* |
Tecan |
| Sciclone* ALH 3000 |
Caliper Life Sciences |
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