Thermo Scientific RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
This RIPA buffer effectively lyses and extracts protein from cultured mammalian cells, including plated cells and pelleted suspension cells. The popular reagent enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the Thermo Scientific BCA Protein Assay, immunoassays and protein purification. Inhibitors such as Thermo Scientific Halt Protease Inhibitor Cocktail (Part No. 78430) and Halt Phosphatase Inhibitor Cocktail (Part No. 78420) are also compatible with this RIPA buffer formulation and can be added before use to prevent proteolysis and maintain protein phosphorylation.
- Convenient – ready-to-use solution; no need to assemble and prepared components yourself
- Flexible – compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification
- Versatile – enables extraction of cytoplasmic, membrane and nuclear proteins
- Disclosed formulation – contains no proprietary components, providing users with complete control and knowledge of possible compatibility issues
RIPA buffer derives its name from the original application for which it was developed: the radio-immunoprecipitation assay. While this isotopic assay method is rarely performed in laboratories today, the acronym for this lysis buffer formulation has endured in common use. RIPA cell lysis reagent is highly effective for protein extraction from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is its relative incompatibility with certain downstream applications compared to other lysis reagents.
|Isolation of cytosolic, nuclear and membrane proteins using RIPA buffer. Total proteins (7µg each) obtained from HeLa or A431 cells were separated on a 4-12% SDS-PAGE gels. Proteins were then transferred to nitrocellulose membrane and probed for the presence of flotillin (membrane), nucleoporin (nuclear) and HSP90 (cytosolic) proteins using western blotting and SuperSignal West Pico chemiluminescent Substrate (Part No. 34080). These results indicate that proteins from all the three different compartments of the cell were readily detected in cell lysates prepared using the Thermo Scientific Pierce RIPA buffer.
Pierce IP Lysis Buffer
M-PER Mammalian Protein Extraction Reagent
Pierce Co-Immunoprecipitation Kit