Western Blotting Substrates

Chemiluminescent (Pierce ECL and SuperSignal) as well as precipitating (colorimetric) substrates for alkaline phosphatase (AP) and horseradish peroxidase (HRP) Western blot detection.

Selection Guides – Choose a substrate for your experiment:
- Chemiluminescent Western Blot Substrate Selection Guide
- Guide to enzyme substrates for Western blotting (pdf)
Protein Methods Library – Learn more about Western blotting substrates:
Related Videos – Watch how methods are performed:
- Western Blot Video Tutorials
Related Product Categories – Other products of interest:
- Western Blotting Kits (including DyLight Fluorescent Kits)


	Pierce ECL Western Blotting Substrate Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.


	Pierce ECL Plus Western Blotting Substrate Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.


	SuperSignal West Pico Chemiluminescent Substrate Enhanced chemiluminescent (ECL) HRP substrate that provides low-picogram-level detection by Western blot analysis.


	SuperSignal West Dura Chemiluminescent Substrate Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.


	SuperSignal West Femto Chemiluminescent Substrate Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.


	Lumi-Phos WB Chemiluminescent Substrate for AP Luminol-based ECL chemiluminescent substrate for alkaline phosphatase (AP, alk-phos) detection in Western blotting applications.


	1-Step TMB-Blotting Substrate Solution Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.


	1-Step Ultra TMB Blotting Solution A non-fading, dark blue, precipitating horseradish peroxidase substrate for chromogenic Western blot detection on nitrocellulose or PVDF membrane.


	Chloronaphthol Substrates Powders, tablets and ready-made 4-chloro-1-naphthol solutions for chromogenic HRP detection in Western blot and tissue staining methods.


	CN/DAB Substrate Kit Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.


	DAB Substrate Kit Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.


	NBT/BCIP Substrates and Solutions Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.