Thermo Scientific Pierce 96-well Spin Plates are designed for high-throughput chromatography applications. These multi-well plates are adapted from traditional chromatography spin-columns to allow the simultaneous processing of up to 96 samples.
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Order Free Literature...
Protein Purification Technical Handbook
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Highlights
- Fast – optimized for high-throughput sample processing
- Compatible – use with automated plate handling systems or common centrifuges
- Reproducible – low well-to-well yield variance
- Ready – simply remove the sealing tape and add your sample
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Learn more...
Protein Extraction
Affinity Purification
Dialysis, Desalting and Buffer Exchange
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Details
Zeba 96-well Spin Plates have two components: a deep-well plate packed with chromatography resin and a collection plate to retain liquids that pass through the resin bed. The deep-well plate comprises a packed chromatography resin bed on top of a polyethylene filter. Pre-packed filter plates are sealed on both the top and bottom of the plate to prevent the resin bed from drying out and avoid contamination during storage. The bottom of each well on the filter plate has a drip director (elution spout) to channel liquids into specific wells of the collection plate.
Multiple samples can be added to the filter plate using standard single or multi-channel pipettes or through common high-throughput automated equipment. Once loaded, the samples can be processed through the chromatography resin bed through a variety of approaches, including centrifugation using swinging-bucket rotors that hold standard 96-well plates or by vacuum or positive-pressure filtration.
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Applications
Zeba 96-well spin plates are commonly used for three types of applications: recombinant protein purification, antibody purification and protein sample clean-up. For each application, the 96-well filter plate is filled with an optimized resin volume and used at a centrifuge speed that allows maximum flow-through.
Use the links below to learn about specific filter plate applications:
Protein purification
Immobilized cobalt agarose resin
Immobilized Ni-NTA agarose resin
Immobilized glutathione agarose resin
Antibody screening
Immobilized Protein A agarose resin
Immobilized Protein G agarose resin
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Cobalt Agarose Plates
Ni-NTA Agarose Plates
Glutathione Agarose Plates
Protein A Agarose Plates
Protein G Agarose Plates
Melon Gel Plates
Zeba, 7K MWCO Desalting Plates
Zeba, 40K MWCO Desalting Plates
Detergent Removal Plates
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| Common Specifications of Thermo Scientific Zeba 96-well Filter Plates. |
| Plate dimensions (l x w x h)† |
127.76 x 85.5 x 45mm ± 0.25mm |
| Well depth |
27.11 ± 0.10mm |
| Well diameter |
7 ± 0.10mm |
| Well off-set |
9mm |
| Well volume |
800µL |
| Plate material |
polypropylene |
| Filter material |
polyethylene |
| Filter pore size |
20µm |
| Collection plate maximum volume |
150µL |
| Max centrifuge speed |
up to 1000 x g |
| Suggested balance plate |
use Part No. 45205 |
| † Plate height includes collection plate for total stack height |
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96-well Filter Collection Plates
96-well Spin Balance Plate |
