Fast Western Blotting Selection Guide

Choose these products for faster Western blotting.

Accelerate your research with our special blotter, transfer buffer and detection kits that are optimized for high-speed Western blotting.

Use these products separately to speed up either transfer or detection steps, or combine all of them to achieve the fastest possible Western blotting procedure. Together, these Thermo Scientifc Pierce Fast Western Blot products enable protein-transfer in 10 minutes and chemiluminescent detection in 55 minutes.

Page Contents:

• Faster Western Blot Transfer
• Faster Western Blot Blocking, Probing and Detection

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Overview of Western Blot Methods

Related literature...

Western Blot Handbook and Troubleshooting Guide

Faster Western Blot Transfer

Fast Semi-dry Blotter

The Thermo Scientific Pierce Fast Semi-Dry Blotter (Part No. 88217) effectively transfers proteins to produce Western blot-ready membranes in 10 minutes without the need to purchase special consumables. The design is cost-effective and allows you to transfer protein from four mini-gels simultaneously, all within 7 to 10 minutes. The blotter can also transfer nucleic acid using alternative buffer conditions.

Highlights:

• Fast – transfer protein from gel to membrane in 7 to 10 minutes
• Sensitive – same transfer efficiency as other semi-dry or wet-transfer units
• Economical – no special consumables needed
• Robust – transfer up to four (4) gels at one time
• Versatile – use homemade or pre-cast gels

Fast Semi-dry Transfer Buffer

The Thermo Scientific Pierce Fast Semi-Dry Transfer Buffer (Part No. 35035) works with any semi-dry transfer unit and with numerous pre-cast and homemade SDS-polyacrylamide gel electrophoresis protein gels. The buffer is a 10X solution that does not require cooling or methanol – simply dilute with water and use immediately.

Highlights:

• Optimized – formulated for rapid transfer with any semi-dry blotting unit
• Non-hazardous – no methanol required, eliminating hazardous disposal
• Compatible – use with all major SDS-PAGE gels (Tris-HEPES, Tris-Glycine, Bis-Tris and Tris-HCl), and nitrocellulose or PVDF membranes
• Easy to use – simply dilute the 10X concentrated formula in water

Western Blot Filter Paper

Thermo Scientific Pierce Western Blotting Filter Papers make blotting work easier and better. The pre-cut cotton sheets are effective for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes.

Highlights:

• Use in Western blots for quickly and efficiently transferring proteins from gel to a membrane
• Precut filter paper packs are a convenient companion for the membranes used in mini-gel sized blotting applications
• Use 1 extra thick filter paper per side of the sandwich instead of 2-3 of the standard kind

Faster Western Blot Blocking, Probing and Detection

Fast Western Blot Kits

Thermo Scientific Pierce Fast Western Blot Kits accelerate the Western blotting process with streamlined protocols and optimized reagents that provide accuracy, sensitivity and reliability. The kits reduce hands-on and overall blotting time to approximately one hour using any of the Thermo Scientific Pierce ECL and SuperSignal Chemiluminescent Substrates.

After transferring proteins to a membrane, traditional Western blotting procedures can take more than 4 hours to complete. Our kits provides all the reagents (except your primary antibody) necessary to complete a Western blot in about 55 minutes. The protocol is easy and requires little or no special optimization. Simply use a mouse or rabbit primary antibody at the same concentration used for typical detection with the Pierce ECL or SuperSignal Substrate. Because it is a reagent-based system, there is no investment in equipment or costly consumables to purchase. Our specially formulated blocking buffers and titered detection antibodies accelerate Western blotting by minimizing incubation times without sacrificing sensitivity.

Highlights:

• Fast – all the sensitivity of Pierce ECL or SuperSignal Substrate while saving 3 to 4 hours per blot
• Convenient – no expensive hardware or vacuum required; no clogging issues
• Simple – optimized protocol makes Western blot analysis easier than ever
• Economical – no expensive consumables or extra equipment required
• Stable – kits are stable for 1 year stored at 4°C
• Easy – complete kits contain all components needed to block, probe and develop a blot with your mouse or rabbit primary antibody

Thermo Scientific Pierce
Fast Western Blot Kits:

Pierce ECL Kit
SuperSignal Pico Kit
SuperSignal Dura Kit
SuperSignal Femto Kit

Browse all Western blot reagents and equipment