DyLight 350
Fluorescent dye properties, example data, product guide and references.
|
|
Thermo Scientific DyLight 350 is a derivative of AMCA (Aminomethylcoumarine acetate), which is a blue fluorescent dye commonly used as a contrasting dye for double- and triple-labeling used in a wide array of applications. The UV-excitable DyLight 350 has spectral properties that are very similar to those displayed by AMCA, including identical excitation wavelengths and a large stokes shift. The emission wavelength of DyLight 350 has a 10-nm blue shift over AMCA, though, that provides an advantage in multiplexing with green fluorophores. In addition, DyLight 350 has enhanced solubility over AMCA, allowing for higher incorporation of the dye into the biomolecule of interest.
Page Contents:
|
Learn more...
Fluorescent Probes
View products...
DyLight Fluor Product Guide
|
DyLight 350 Properties and Applications
|
 |
| Thermo Scientific DyLight 350 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (purple) spectra are normalized to the same height in this graph. |
Properties of Thermo Scientific DyLight 350.
| Parameter |
Value |
| Excitation / emission maxima |
353nm / 432nm |
| Emission color |
Blue |
| Molar extinction coefficient (ε) |
15,000 M-1 cm-1 |
| Correction factor (A260/A353) † |
0.144 |
| Molecular weight |
NHS ester: 874g/mol
Maleimide: 899g/mol
|
| Spectrally similar dyes |
Alexa Fluor* 350, AMCA |
| † Correction factor can be used to estimate protein labeling efficiency. |
|
Learn more...
Compare spectra and properties among all DyLight Fluors
|
|
|
Learn more...
Fluorescent Probes: Description, Methods and Applications
Related literature...
Fluorescent Labeling and Detection Products Guide
|
DyLight 350 Comparative Data
|
 |
 |
 |
| Thermo Scientific DyLight 350 brilliant fluorescent staining is substantially brighter than AMCA and evenly comparable to Alexa Fluor* 350. Cytokeratin 18 (blue) was fluorescently labeled in A549 cells with a rabbit polyclonal anti-cytokeratin 18 antibody and goat anti-rabbit IgG secondary antibodies conjugated to AMCA, Alexa Fluor* 350 or DyLight 350. Lamin A (green) was labeled with anti-lamin A antibody and DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibody. All images were captured using identical microscopic and camera settings. |
|
DyLight 350 Products
|
|
|
Related products:
DyLight Specialty Dyes
DyLight Fluor Product Guide
|
