DyLight 488

Fluorescent dye properties, example data, product guide and references.

Thermo Scientific DyLight 488 provides exceptional performance for a wide array of fluorescence-base applications at a lower cost than other 488-range dyes. DyLight 488 shows greater photostability than traditional fluorescein-based dyes and has a greater range of pH insensitivity (pH 4-9). DyLight 488 is available as reactive labeling agents and as conjugates of secondary antibodies and biotin-binding proteins for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.

Page Contents:

• DyLight 488 Properties and Applications
• DyLight 488 Comparative Data
• DyLight 488 Products
• DyLight 488 References

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Fluorescent Probes

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DyLight Fluor Product Guide

DyLight 488 Properties and Applications

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Compare spectra and properties among all DyLight Fluors

DyLight 488 Comparative Data

DyLight 488 Products

DyLight 488 Reactive Dyes and Kits:

• Amine-reactive dye (N-hydroxysuccinimide (NHS) ester-activated)
• Sulfhydryl-reactive dye (maleimide-activated)
• Azide-reactive dye (phosphine-activated)
• Free acid dye – non-reactive; used as negative control

DyLight 488-conjugated Fluorescent Probes:

• Secondary antibody-conjugates – Goat Anti-Mouse or Anti-Rabbit
• Streptavidin-conjugates
• Neutravidin protein-conjugates

DyLight 488 References

1. Ahmed, S.M. et al. (2010) G protein betagamma subunits regulate cell adhesion through Rap1a and its effector Radil. J. Biol. Chem. 285, 6538-51.
2. Bechler, M.E. et al. (2010) The phospholipase complex PAFAH Ib regulates the functional organization of the Golgi complex. J. Cell Biol. 190, 45-53.
3. Brunetti, V. et al. (2010) Neurons sense nanoscale roughness with nanometer sensitivity. Proc. Natl. Acad. Sci. U.S.A. 107, 6264-9.
4. Cai, J. et al. (2010) Generation of homogeneous PDX1(+) pancreatic progenitors from human ES cell-derived endoderm cells. J. Mol. Cell. Biol. 2, 50-60.
5. Charles, S.M. et al. (2010) Roles of cytosolic Ca2+ concentration and myofilament Ca2+ sensitization in age-dependent cerebrovascular myogenic tone. Am. J. Physiol. Heart Circ. Physiol. 299, H1034-44.
6. Chen, M.J. et al. (2010) Development and characterization of five rainbow trout pituitary single-cell clone lines capable of producing pituitary hormones. J. Endocrinol. 205, 69-78.
7. Chou, W.-H. et al. (2010) GABAA receptor trafficking is regulated by protein kinase C{varepsilon} and the N-ethylmaleimide-sensitive factor. J. Neurosci. 30, 13955-65.
8. Cordell, P.A. et al. (2010) Association of coagulation factor XIII-A with Golgi proteins within monocyte-macrophages: implications for subcellular trafficking and secretion. Blood 115, 2674-2681.
9. Farrar, D. et al. (2010) Mutational analysis of the poly(ADP-ribosyl)ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly(ADP-ribosyl)ation. Mol. Cell. Biol. 30, 1199-216.
10. Guo, F. et al. (2010) Pyramidal neurons are generated from oligodendroglial progenitor cells in adult piriform cortex. J. Neurosci. 30, 12036-49.
11. Hagedorn, I. et al. (2010) Factor XIIa inhibitor recombinant human albumin Infestin-4 abolishes occlusive arterial thrombus formation without affecting bleeding. Circulation 121, 1510-7.
12. Hudson, D.M. et al. (2010) Human hephaestin expression is not limited to enterocytes of the gastrointestinal tract but is also found in the antrum, the enteric nervous system, and pancreatic {beta}-cells. Gastroint. Liver Physiol. 298, G425-32.
13. Ihara, Y. et al. (2010) C-Mannosylated peptides derived from the thrombospondin type 1 repeat interact with Hsc70 to modulate its signaling in RAW264.7 cells. Glycobiology 20, 1298-1310.
14. Kirschman, L.T. et al. (2010) The Leber congenital amaurosis protein, AIPL1, is needed for the viability and functioning of cone photoreceptor cells. Hum. Mol. Genet. 19, 1076-87.
15. Kleinschnitz, C. et al. (2010) Early detrimental T-cell effects in experimental cerebral ischemia are neither related to adaptive immunity nor thrombus formation. Blood 115, 3835-42.
16. Knowles, H.J. et al. (2010) Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4. FASEB J.
17. Koduru, S. et al. (2010) Cdc42 interacting protein 4 (CIP4) is essential for integrin-dependent T-cell trafficking. Proc. Natl. Acad. Sci. U.S.A. 107, 16252-56.
18. Koya, R.C. et al. (2010) Kinetic phases of distribution and tumor targeting by T cell receptor engineered lymphocytes inducing robust antitumor responses. Proc. Natl. Acad. Sci. U.S.A.
19. Leffler, J. et al. (2010) Annexin-II, DNA, and histones serve as factor H ligands on the surface of apoptotic cells. J. Biol. Chem. 285, 3766-76.
20. Lei, X., et al. (2010) The 3C Protein of Enterovirus 71 Inhibits Retinoid Acid-Inducible Gene I-Mediated Interferon Regulatory Factor 3 Activation and Type I Interferon Responses. J. Virol. 84, 8051-61.
21. Merkel, V. et al. (2010) Distribution and phylogeny of immunoglobulin-binding protein G in Shiga Toxin-producing Escherichia coli and its association with adherence phenotypes. Infect Immun. 78, 3625-36.
22. Panchal, R.G. et al. (2010) Development of high-content imaging assays for lethal viral pathogens. J. Biomol. Screen. 15, 755-65.
23. Pleines, I. et al. (2010) Multiple alterations of platelet functions dominated by increased secretion in mice lacking Cdc42 in platelets. Blood 115, 3364-73.
24. Qu, Y. et al. (2010) Atypical cadherins Celsr1-3 differentially regulate migration of facial branchiomotor neurons in mice. J. Neurosci. 30, 9392-401.
25. Schaff, U.Y. et al. (2010) Neutrophils exposed to A. phagocytophilum under shear stress fail to fully activate, polarize, and transmigrate across inflamed endothelium. Am. J. Physiol. Cell Physiol. 299, C87-96.
26. Schaff, U.Y. et al. (2010) Orai1 regulates intracellular calcium, arrest, and shape polarization during neutrophil recruitment in shear flow. Blood 115, 657-66.
27. Song, X. et al. (2010) Inhibition of retinoblastoma in vitro and in vivo with conditionally replicating oncolytic adenovirus H101. Invest. Ophthalmol. Vis. Sci. 51, 2626-35.
28. Thompson, E.D. et al. (2010) Tumor masses support naive T cell infiltration, activation, and differentiation into effectors. J. Exp. Med. 207, 1791-804.
29. Tu, Z. et al. (2010) Tissue distribution and functional analysis of sushi domain-containing protein 4. Am. J. Pathol. 176, 2378-84.
30. Vaidyanathan, R. et al. (2010) Regulation of cardiac inward rectifier potassium current (IK1) by synapse associated protein-97. J. Biol. Chem. 285, 28000 -9.
31. Wang, C., Pan, Y. & Wang, B. Suppressor of fused and Spop regulate the stability, processing and function of Gli2 and Gli3 full-length activators but not their repressors. Development 137, 2001-9.
32. Wang, W.-X. et al. (2010) miR-107 regulates granulin/progranulin with implications for traumatic brain injury and neurodegenerative disease. Am. J. Pathol. 177, 334-45.
33. Yang, J. et al. (2010) Kupfer-type immunological synapse characteristics do not predict anti-brain tumor cytolytic T-cell function in vivo. Proc. Natl. Acad. Sci. U.S.A. 107, 4716-21.
34. Zhou, C. et al. (2010) {alpha}1G T-type calcium channel selectively regulates P-selectin surface expression in pulmonary capillary endothelium. Am. J. Physiol. Lung. Cell Mol. Physiol. 299, L86-97.

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