Standard and extra-thick filter paper for protein transfer blotting.
Thermo Scientific Pierce Western Blotting Filter Papers are pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes.
Sheets of blotting paper are necessary components of transfer sandwiches and cassettes that typically must be assembled for various kinds of protein or nucleic acid, gel-to-membrane, transfer protocols. High-quality Pierce Western Blotting Filter Papers are available as standard-thickness and extra-thick sheets that are pre-cut for convenience with mini gels.
High-quality – clean cotton cellulose fiber paper manufactured with additive-free ultrapure water to eliminate sources of background signal and artifacts
Easy to use – pre-cut filter paper sheets in several convenient sizes for use with most mini gel sizes, tank transfer cassettes and semi-dry blotters
Two thicknesses – choose standard-thickness paper for traditional procedures or extra-thick filter paper for high capacity blotting or to eliminate using multiple sheets
Validated – tested and recommmend for use with protein methods, including the Thermo Scientific Pierce Fast Semi-Dry Blotter (Part No. 88217)
Pierce Western Blotting Filter Papers are composed of high-quality alpha cotton cellulose fiber for use in Northern, Southern, and Western blotting applications. These blotting papers help to ensure quick and efficient transferring molecules from a gel to membrane of choice. These filter papers are manufactured with ultrapure water without additives to minimize possible sources of background signal.
Pierce filter papers are suitable for use with alcohol or other organic solvents commonly used in protein and nucleic acid blotting applications. Two thicknesses and several pre-cut sizes provide for optimal wicking, drying and blotting properties. The advantage of using extra-thick filter paper is that only one sheet is needed per side of a typical transfer-sandwich and transfer-casette instead of the usual two or three sheets per side. The extra-thick filter paper is recommended for use with the Pierce Fast Semi-Dry Blotter (Part No. 88217).