For samples prepared for 1-D or 2-D electrophoresis.
The Thermo Scientific Compat-Able Protein Assay Preparation Set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
The Compat-Able Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 minutes with just a few simple steps. The Compat-Able Preparation Set is available individually or conveniently bundled with either the Thermo Scientific Pierce BCA Protein Assay Kit or the Thermo Scientific Pierce Coomassie Plus Protein Assay Kit.
Highlights:
Eliminate incompatibilities – removes nearly any substance that interferes with colorimetric protein assays: salts, detergents, denaturants, reducing agents, and even IEF sample buffer
Simple and fast – prepare samples in less than 10 minutes with four simple steps
Convenient – reagent set is easier to use than homemade TCA or acetone precipitation reagents, thereby producing more consistent results
Scalable – microcentrifuge method is easily scaled to larger centrifuge tube volumes for special needs
Validated – tested and offered together with BCA and Coomassie Plus Protein Assay Kits; protocol integrates control and normalization procedures
Product Details:
Protocol summary for the Pierce Compat-Able Protein Assay Preparation Reagent Set.
Ordering Information
Product #
Description
Pkg. Size
Instructions
MSDS
CofA
Price
23215
Compat-Able Protein Assay Preparation Reagent Kit
Formulation: Proprietary reagent solutions Sufficient For: Treating up to 500 protein assay samples Kit Contents: Compat-Able Reagent 1, 250mL Compat-Able Reagent 2, 250mL
500mL kit
$103.00
23229
Compat-Able BCA Protein Assay Kit
Formulation: Multi-component kit Sufficient For: Treating up to 500 protein assay samples and performing 250 tube assays or 2500 microplate assays Kit Contents: Compat-Able Reagent 1, 250mL Compat-Able Reagent 2, 250mL BCA Reagent A, 500mL BCA Reagent B, 25mL Albumin Standard Ampules, 2mg/mL, 10 x 1mL
1L kit
$188.00
23239
Compat-Able Coomassie Plus Protein Assay Kit
Formulation: Multi-component kit Sufficient For: Treating up to 500 protein assay samples and performing 630 tube assays or 3160 microplate assays Kit Contents: Compat-Able Reagent 1, 250mL Compat-Able Reagent 2, 250mL Pierce Coomassie Plus Assay Reagent, 950mL Albumin Standard Ampules, 2mg/mL, 10 x 1mL