Thermo Scientific Pierce Protein Precipitation Plates provide an easy, 96-well format for simple, rapid and automatable precipitation and removal of proteins from plasma, serum and other biological fluid samples.
These 96-well filtration plates are designed specifically for solvent-based (e.g., acetonitrile) precipitation of proteins and their subsequent removal from fluid samples. The plates are leak-free and retain organic solvent without dripping, even while mixing. Samples are rapidly recovered by applying vacuum or other pressure. The 2mL well volume allows processing sample volumes of 15 to 400mL per well, using the recommended precipitation conditions of 3:1 (v/v) solvent-to-serum ratio. The Protein Precipitation Plates are the ideal choice for downstream processing of plasma protein binding assay samples prepared with equilibrium dialysis systems.
- Prepare small molecule samples for HPLC or LC-MS in 10-30 minutes
- 96-well format for automatable protein precipitation and filtration
- Optimized for parallel processing and automation
- Works seamlessly with the Rapid Equilibrium Device (RED) for plasma protein-binding assays
- No acetonitrile leakage for up to four hours
- Leach-free graded hydrophobic frit prevents blockage
- Filtrates are easily collected by vacuum, positive pressure or centrifugation
- Compatible with most organic solvents
The design of Pierce Protein Precipitation Plates provides many advantages over standard protein precipitation methods based solely on either filtration or centrifugation. The plates use a special graded filter system that retains precipitated protein particles without clogging, yet freely allows soluble (nonprecipitated) eluates through the 0.2µm cut-off membrane. This design maximizes protein removal and ensures the highest possible level of sample recovery.
Thermo Scientific Pierce Protein Precipitation Plate protocol summary.
The study of small molecules and how they behave in complex biofluids (serum, plasma, blood and urine) is an essential part of pharmaceutical, DMPK and metabolomics research. Drugs or other small molecules are routinely monitored for clearance rates, steady-state concentrations, serum-binding equilibrium or in vivo modification. These studies require isolation of target molecules from the protein matrix before analysis. Precipitating proteins with acetonitrile and then removing the precipitate by centrifugation or filtration is the typical isolation process. Although centrifugation to form pellets is inexpensive and reliable, 96-well filtration plates provide an easy format for reproducible, simple, rapid and automatable protein precipitation and sample clarification.