Human IFN beta ELISA Kit

Assay IFN-β (human interferon beta) levels in human cell and tissue culture media (supernatants) with these VeriKine* Human IFN beta ELISA Kits.

These human IFN beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to accurately measure interferon beta (IFN-β) in samples at 100μL per well. These IFN-β kits are validated for use with human cell and tissue culture media. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:

• Target: human interferon beta (human IFN-β, IFN-beta)
• Format: colorimetric ELISA (TMB substrate) in clear 96-well plate
• Measurement: absorbance at 450nm
• Assay Range: 25 to 2000pg/mL
• Sensitivity: undetermined
• Precision: intra-assay CV ≤ 10%; inter-assay CV ≤10%
• Sample Types: cell culture supernatant, tissue culture media
• Total Assay Time: 3.25 hours
• Sample Size: 100μL
• Specificity: no significant cross-reaction with human IFN-α or IFN-γ; mouse or rat IFN-β.

Includes:

Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, substrates, and stop solution.

Details:

Human IFN-β ELISA standard curve. An example standard curve for the VeriKine* Human IFN-β ELISA Kit (Part No. 414101), with error bars based on three replicates for each interferon beta standard concentration.

Related Resources:

• Review of ELISA

Related Products:

• Reagent Reservoirs
• Search IFN beta Antibodies (www.pierce-antibodies.com) – matched pair links coming soon