Creates 8-angstrom crosslinks between amines while maintaining overall charge properties.
Thermo Scientific Pierce DMA is dimethyl adipimidate, a membrane-permeable crosslinker that contains an amine-reactive imidoester group at each end of a 6-atom spacer arm.
Highlights:
Water-soluble
Reactive groups: imidoester (homobifunctional)
Reactive towards: amino groups
Rapid reaction with amines at alkaline pH values (pH 8-10)
Amidine bond retains net charge character of protein
Reversible at high pH values
Tool for study of quarternary structure of proteins
Retains charge character
Product Details:
References:
Dihazi, G.H. and Sinz, A. (2003). Rapid Commun. Mass Spectrom. 17, 2005-2014.
Hartman, F.C. and Wold, F. (1967). Biochemistry 6(8), 2439-2448.
Pearson, K.M., et al. (2002). Rapid Commun. Mass Spectrom. 16, 149-159
Ordering Information
Product #
Description
Pkg. Size
Instructions
MSDS
CofA
Price
20660
DMA
Formulation: Dimethyl adipimidate-2HCl; powder-granule solid Sufficient For: 4079mL of typical 1X (1mM) reaction solution, or approx. 500 uses at 2mg reagent per use
