Thermo Scientific Sodium Cholate is an ionic detergent that is especially useful for liposome preparation and lipid isolation.
Sodium cholate (cholic acid) is a water-soluble, bile-acid commonly used in protein methods such as cell lysis, liposome preparation, isolation of membrane proteins and lipids, preventing nonspecific binding in affinity chromatography and as a cell culture media supplement.
Highlights:
- Anionic, bile-acid detergent for many laboratory uses
- Effective for liposome preparation and lipid isolation
- Can be removed from solution by dialysis
- High-purity compound with low UV absorptivity
Product Details:
At concentrations above 2mM, cholate will form micelles having MW ~900 to 1200. The small size of these micelles allows them to be easily remove by dialysis or gel filtration if needed. Note: removal of a detergent from a protein solution my result in protein precipitation and/or aggregation.
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Chemical structure of sodium cholate detergent.
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Properties of Sodium cholic acid:
- Alternative Names: Sodium cholic acid; cholate, sodium salt
- Chemical Name: 3,7,12-Trihydroxy-5β-cholan-24-oic acid, monosodium salt
- Molecular Weight: 430.6g
- Detergent Class: Ionic (anionic)
- Aggregation Number: 2 to 4 (average)
- Micelle Molecular Weight: 900g (average)
- Critical Micelle Concentration (CMC): 9 to 14mM (0.388 to 0.603% w/v)
- Cloud Point: Unknown
- Dialyzable: Yes
Specifications for Sodium Cholate (Part No. 89906, 89907):
- Formula: C24H39O5Na
- Molecular Weight: 430.6g
- Purity (by HPLC): ≥97%
- Absorbance (1% Detergent Solution): 400nm <0.02; 340nm <0.04; 280nm <0.08; 260nm <0.1
- pH (1% Solution): 5 to 8
- Solubility (in water at 20°C): ≥40%
Related Resources:
Review of Detergents for Cell Lysis
Related Products:
Detergent Sampler Pack
Other Detergents for Protein Methods
Detergent Removal Resins
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