n-Dodecyl-beta-D-Maltoside

Thermo Scientific n-Dodecyl-β-D-Maltoside is especially useful for solubilizing membrane proteins to preserve their activity.

The water soluble nonionic detergent is most often used for the isolation of hydrophobic membrane proteins. Multiple studies have shown that n-Dodecyl-β-D-maltoside is a gentle detergent that is often able to preserve protein activity better than many commonly used detergents, including NP-40, CHAPS and Octyl-β-glucoside.

Highlights:

• Lipid-like nonionic detergent
• Especially useful for isolating and stabilizing hydrophobic membrane proteins
• Preserves activity of membrane protein better than most of the detergents
• High-purity compound with low UV absorptivity

Product Details:

Like most detergents, n-Dodecyl-β-D-maltoside has dual hydrophobic/hydrophilic properties that facilitate lipid displacement and provide a lipid-like environment for membrane proteins. Studies suggest that the ability of these surfactants to preserve membrane protein structure stems in part from the reduced disruption of lipid:protein interactions where some of the natural lipid associations are maintained. While no detergent or set of conditions is optimal for all studies on membrane proteins, n-Dodecyl-β-D-maltoside has unique characteristics that have made it especially helpful in certain protein methods.

Chemical structure of n-Dodecyl-beta-D-maltoside detergent.

Properties of n-Dodecyl-β-D-Maltoside:

• Chemical Name: n-Dodecyl-beta-D-maltoside
• Molecular Weight: 510.6g
• Detergent Class: Nonionic
• Aggregation Number: 98 (average), 70 to 140 range
• Micelle Molecular Weight: 50,000g
• Critical Micelle Concentration (CMC): 0.17mM (0.009%, w/v) in water ; 0.12mM (0.006%, w/v) in 0.2M NaCl
• Cloud Point: Unknown
• Dialyzable: No

Specifications for n-Dodecyl-beta-maltoside (Part No. 89902, 89903):

• Formula: C₂₄H₄₆O₁₁
• Molecular Weight: 510.6g
• Purity (by HPLC): ≥99%
• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06; 225nm <0.1
• pH (1% Solution): 5 to 8
• Solubility (in water at 0 to 5°C): ≥20%
• Conductivity (10% Solution) : <40mS

References:

1. Casey, J. R.., et al. (1993). Biochemistry, 32, 1172-1179.
2. Engel, C.K., et. al. (2002). Biochimica et Biophysica Acta, 1564, 47-56.
3. Boulter, J. M., Wang, D. N., (2001). Protein Expression and Purification, 22, 337-348.
4. le Maire, M., et. al. (2000). Biochimica et Biophysica Acta, 1508, 86-111.
5. Banerjee, P., et. al. (1995). Chemistry and Physics of Lipids, 77, 65-78.
6. Wang, D. N., et. al. (2003). Biochimica et Biophysica Acta, 1610, 23-36.
7. Berger,B. W., et. al. (2005). Biophysical Journal, 89, 452-464.
8. Auer, M. et. al. (2001). Biochemistry, 40, 6628-6635.

Related Resources:

Review of Detergents for Cell Lysis

Related Products:

Detergent Sampler Pack
Other Detergents for Protein Methods
Detergent Removal Resins