Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive visualization with fluorescence imagers (520nm excitation; 580nm emission) of proteins separated in polyacrylamide gels or transferred to membranes.
This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis (SDS-PAGE). Krypton Stain uses a protein-binding dye with fluorescence properties (Ex/Em = 520/580nm) suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane (greater than 2ng for the rapid staining protocol). Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.
- Intense fluorescent stain – excitation and emission maxima of 520nm and 580nm, respectively (compatible with 532nm excitation light sources and 580 to 600nm emission filters)
- Instrument-compatible – effective with popular laser-based and filter-based fluorescence imagers
- Broad dynamic range – linear quantitative range spans three to four orders of magnitude Sensitive – detect as little as 0.25ng protein with the standard protocol (approx. 2.5 hours); at least as sensitive as fluorescent stains from other suppliers
- Fast – standard (2.5 hours) and rapid (30 minutes) protocols are significantly faster than procedures of other popular fluorescent stains
- Versatile – compatible with proteomics workflows involving downstream mass spectrometry
- Compact – supplied as a 10X solution to save storage space; just dilute with water before use
Product Details:
For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.
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| Thermo Scientific Krypton Protein Stain is fast and sensitive. Proteins were separated in 4-20% Tris-glycine gels and stained using the indicated protocols. The gels were imaged with the Typhoon* 9410 Imager at 532nm excitation and 580 BP30 emission. |
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| Faster than other commercial fluorescent gel stains. Thermo Scientific Krypton Fluorescent Protein Stain works up to five times faster than other fluorescent stain protocols in both rapid and basic procedures. |
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| Sensitivity equal to or greater than other fluorescent stains in 2D gel analysis. The Thermo Scientific Krypton Fluorescent Protein Stain works well for 2D gels. Processed HeLa cell protein extract (28µg) was focused on a pH 5-8 IPG strip followed by 4-20% SDS-PAGE. The gels were stained and imaged following manufacturer-recommended basic protocols. |
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| Thermo Scientific Krypton Protein Stain produces a linear response to staining with minimal protein-to-protein variation. Relative fluorescent intensity was plotted as a function of protein quantity for proteins of various sizes and containing post-translational modifications. |
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| Thermo Scientific Krypton Protein Stain exhibits a more linear quantitative range than other fluorescent stains. Relative fluorescent intensity for each protein concentration is the average of nine different proteins separated in 4-20% Tris-glycine and Precise Gels. Error bars represent the standard deviation for triplicate gels. The gels were stained and imaged according to each manufacturer’s basic protocols. |
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