DyLight 650
Fluorescent dye properties, example data, product guide and references.
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Thermo Scientific DyLight 650 replaces DyLight 649 to provide vibrant far-red fluorescence with comparable or improved performance compared to other dyes, including Alexa Fluor* 647 and Cy5*, for fluorescent applications. DyLight 650 dye has tight excitation/emission spectra that are ideal for multi-color applications. DyLight 650 dye is available as reactive labeling agents for custom labeling applications. DyLight 650-conjugated secondary antibodies and biotin-binding proteins are also available for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.
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Fluorescent Probes
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DyLight Fluor Product Guide
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DyLight 650 Properties and Applications
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| Thermo Scientific DyLight 650 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (red) spectra are normalized to the same height in this graph. |
Properties of Thermo Scientific DyLight 650.
| Parameter |
Value |
| Excitation / emission maxima |
652nm / 672nm |
| Emission color |
Far Red |
| Molar extinction coefficient (ε) |
250,000 M-1 cm-1 |
| Correction factor (A260/A652) † |
0.037 |
| Molecular weight |
NHS ester: 1066g/mol
Maleimide: 1091g/mol
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| Spectrally similar dyes |
Alexa* Fluor 647, Cy5 |
| † Correction factor can be used to estimate protein labeling efficiency. |
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Compare spectra and properties among all DyLight Fluors
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| Immunofluorescence microscopy using Thermo Scientific DyLight 650 dye. Intermediate filaments (red) in A549 cells were fluorescently labeled with biotinylated mouse anti-cytokeratin 18 antibody and DyLight 650-Conjugated Streptavidin. Nuclei were labeled with Hoechst dye.Top panel: Cells were imaged on the Thermo Scientific ArrayScan VTI HCS Reader. Bottom panel: Cells were images with a Zeiss* Axio Observer Z1* epifluorescent microscope. |
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Fluorescent Probes: Description, Methods and Applications
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Fluorescent Labeling and Detection Products Guide
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DyLight 650 Comparative Data
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| Thermo Scientific DyLight 650 dye produces intense fluorescent staining that is comparable to our retired DyLight 649 dye and brighter than other commercially available dyes. The nuclear envelope in A549 cells was fluorescently labeled with anti-lamin A (top row) or anti-lamin B1 (bottom row) primary antibodies and commercially available goat anti-mouse or anti-rabbit IgG secondary antibodies, respectively, that were conjugated to DyLight 650, the retired DyLight 649, Alexa Fluor 647 or Cy5 dye. All images were captured using identical microscopic and camera settings and were processed identically. |
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| Thermo Scientific DyLight 650 produces intense vimentin staining that is brighter than other commercially available dyes. Intermediate filaments in U2OS cells were fluorescently labeled with a monoclonal anti-vimentin antibody and commercially available goat anti-mouse IgG secondary antibody conjugated to Thermo Scientific DyLight 650, Alexa Fluor 647 or Cy5 dye. Nuclei were counterstained with Hoechst dye. All images were captured using identical microscopic and camera settings and were processed identically. |
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DyLight 650 Products
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Related products:
DyLight Specialty Dyes
DyLight Fluor Product Guide |
