DyLight 550
Fluorescent dye properties, example data, product guide and references.
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Thermo Scientific DyLight 550 replaces DyLight 549 to provide vibrant orange-to-red fluorescence with better performance than other rhodamine derivatives, including Alexa Fluor* 555, TRITC and Cy3* for fluorescent applications. DyLight 550 has tight excitation/emission spectra that are ideal for multicolor applications. DyLight 550 is available as reactive labeling agents for custom labeling applications. DyLight 550-conjugated secondary antibodies and biotin-binding proteins are also available for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.
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Fluorescent Probes
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DyLight Fluor Product Guide
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DyLight 550 Properties and Applications
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| Thermo Scientific DyLight 550 dye spectra. Fluorescent dyes are named based on their excitation (absorption) maxima. The excitation (black) and emission (orange) spectra are normalized to the same height in this graph. |
Properties of Thermo Scientific DyLight 550.
| Parameter |
Value |
| Excitation / emission maxima |
562nm / 576nm |
| Emission color |
Orange to Red |
| Molar extinction coefficient (ε) |
150,000 M-1 cm-1 |
| Correction factor (A260/A562) † |
0.081 |
| Molecular weight |
NHS ester: 1040g/mol
Maleimide: 1065g/mol
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| Spectrally similar dyes |
Alexa Fluor* 555, Cy3, TRITC |
| † Correction factor can be used to estimate protein labeling efficiency. |
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Compare spectra and properties among all DyLight Fluors
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| Immunofluorescence microscopy using Thermo Scientific DyLight 550 dye. Top panel: Protein disulfide isomerase (PDI; red) in U2OS cells was detected with an anti-PDI primary antibody and goat anti-mouse secondary antibody labeled with the DyLight 550 Microscale Antibody Labeling Kit. Nuclei were labeled with Hoechst dye. Bottom panel: Cytokeratin 18 (green) and lamin B1(red) in U2OS cells were detected with the respective primary antibodies and goat anti-mouse and anti-rabbit secondary antibodies labeled with DyLight 550 and 650 Microscale Antibody Labeling Kits, respectively. |
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Fluorescent Probes: Description, Methods and Applications
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Fluorescent Labeling and Detection Products Guide
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DyLight 550 Comparative Data
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| Thermo Scientific DyLight 550 dye produces intense fluorescent staining that is comparable to our retired DyLight 549 dye and brighter than other commercially available dyes. The nuclear envelope in A549 cells was fluorescently labeled with anti-lamin A (top row) or anti-lamin B1 (bottom row) primary antibodies and commercially available goat anti-mouse or anti-rabbit IgG secondary antibodies, respectively, that were conjugated to Thermo Scientific DyLight 550, the retired DyLight 549, Alexa Fluor 555 or Cy3 dye. All images were captured using identical microscopic and camera settings and were processed identically. |
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| Thermo Scientific DyLight 550 dye produces intense NF-kB staining that is comparable to our retired DyLight 549 dye and brighter than other commercially available dyes. NF-kB (orange) in A549 cells was fluorescently labeled with an anti-NFkB primary antibody and a commercially available goat anti-mouse IgG secondary antibody conjugated to DyLight 550, the retired DyLight 549, Alexa Fluor 555 or Cy3 dye. Nuclei (blue) were stained with Hoechst dye. All images were captured using identical microscopic and camera settings and were processed identically. |
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DyLight 550 Products
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Related products:
DyLight Specialty Dyes
DyLight Fluor Product Guide |
