The Thermo Scientific Pierce In-Solution Tryptic Digestion and Guanidination Kit contains proteomics-grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
Accurate identification of proteins and analysis of post-translational modifications by mass spectrometry require accurate and complete protein digestion and peptide modification. The In-Solution Tryptic Digestion and Guanidination kit provides an optimized procedure and reagents for approximately 90 digests, each containing 0.025 to 10µg of protein.
Highlights:
- Optimized trypsin digestion – produce complete digests of protein ranging from 0.025 to 10µg
- Optimized guanidination – reaction produces little to no N-terminal modification
- Convenient – kit includes reagents for digestion, reduction, alkylation and guanidination.
- Quick – protein can be digested and guanidinated all in one day
Product Details:
Trypsin specifically cleaves peptide bonds at the carboxyl side of arginine and lysine residues. The signals from the arginine-containing peptides are generally stronger, due to the more basic side chain. To enhance overall ionization, guanidination is necessary to convert lysines to homoarginines. The guanidination reaction is specific for the amine of lysine but may occur minimally at the amine of the peptide's N-terminus. This derivatization leads to an increase in the intensity of the lysine containing peptides and improved sequence coverage overall.
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Procedure summary for the Thermo Scientific In-Solution Tryptic Digestion and Guanidination Kit.
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| Guanidination increases sequence coverage. Significantly greater numbers of peptides are identified from a variety of test proteins after guanidination (also called guanidinylation). Peptides identified determined based on scanning from 600 to 2000m/z. |
| Protein |
Sequence Coverage
No Guanidination |
Sequence Coverage
With Guanidinated |
| Lysozyme (14kDa) |
6/8 peptides
66/88 aa
77% |
8/8 peptides
86/86 aa
100% |
| Myoglobin (17kDa) |
6/12 peptides
78/134 aa
58% |
8/12 peptides
90/134 aa
67% |
| BSA (66kDa) |
25/44 peptides
318/489 aa
65% |
38/44 peptides
344/489 aa
70% |
Related Products:
DTT (dithiothreitol)
In-Gel Tryptic Digestion Kit
Pierce Trypsin, MS Grade
Proteases for Mass Spectrometry
Pierce C-18 Spin Columns
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