Microplate kits and reagent sets for sandwich ELISA of interleukin-1 beta (IL-1b).
Assay IL-1β (human interleukin-1 beta) levels in human serum, plasma (heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-1 beta ELISA Kits.
These human IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-1β antibody pairs and IL-1β standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant human interleukin-1 beta (IL-1β)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with human IL-1α, IL-1RA, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, IFNα, IFNγ or mouse IL-1β
†Note: Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).
Human IL-1β ELISA standard curve. An example standard curve for the Thermo Scientific Human IL-1β ELISA Kit (Part No. EH2IL1B), with error bars based on four replicates for each interleukin-1 beta standard concentration. Also see standard curves for the IL-1β Reagent Kit and IL-1β Antibody Pair Kit.