Thermo Scientific Pierce Streptavidin Plus UltraLink Resin is a beaded polyacrylamide resin of immobilized recombinant streptavidin protein for use in a variety of biotin-binding affinity purification methods.
Pierce Streptavidin Plus UltraLink Resin consists of purified recombinant streptavidin that has been covalently immobilized at high density onto a beaded acrylamide support. Streptavidin is a tetrameric biotin-binding protein. UltraLink beads are hydrophilic, charge-free, highly crosslinked, rigid and durable. These properties make the affinity resin suitable for a variety of batch- or column-type affinity procedures that require medium-pressure chromatography. The resin provides an alternative to agarose supports when situations demand it. Biotinylated antibodies, proteins, peptides, nucleic acids and other molecules or interaction complexes can be captured, immunoprecipitated, removed or purified from samples using this streptavidin resin.
Highlights:
- Streptavidin – purified recombinant streptavidin protein (53kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
- UltraLink Resin – acrylamide-based support provides an alternative to crosslinked beaded agarose supports for medium-pressure applications
- High capacity – this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity of at least 3mg of biotinylated BSA per mL of resin
Applications:
- Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies
- Isolation of cell-surface proteins that have been labeled with a cleavable biotinylation reagent
- Isolation of cell-surface glycoproteins using biotinylated Concanavalin A
- Recovery of single-stranded biotinylated DNA for dideoxy sequencing
- Streptavidin-coated plates used for direct solid-phase sequencing
- In vitro mutagenesis with streptavidin-coated plates
- DNA fingerprinting with streptavidin-coated plates
Product Details:
Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Thermo Scientific Pierce Streptavidin Resins has a near-neutral isoelectric point and a mass of 53kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.
UltraLink Resin is an azlactone-activated polyacrylamide support that is hydrophilic, charge-free, high capacity, highly crosslinked, rigid and porous. This support is especially useful for medium pressure techniques when using large sample volumes requiring fast-flow techniques (FPLC) and large-scale applications. Unlike typical beaded agarose supports, UltraLink Support does not crush or collapse when subjected to high flow rates that result in pressures exceeding 25psi.
Comparison of available Thermo Scientific Pierce Streptavidin Resins. Binding-capacity specifications (Capacity) are expressed as milligrams of biotinylated BSA bound per milliliter of resin. Store all resins in water or buffer at 4°C; do not freeze. Also consider our NeutrAvidin Resins.
| Product |
Support |
Capacity |
Available Formats |
Streptavidin
Agarose Resin |
Crosslinked 6% beaded agarose |
1 to 3mg |
Bulk resin (2, 5, 10mL);
Spin columns (1mL resin) |
High Capacity
Streptavidin
Agarose Resin |
Crosslinked 6% beaded agarose |
>10mg |
Bulk resin (2, 5, 10mL);
Chromatography cartridges (1, 5mL) |
Streptavidin
UltraLink Resin |
Co-polymer of crosslinked bisacrylamide and azlactone |
>2mg |
Bulk resin (2, 5mL) |
| Streptavidin Plus UltraLink Resin |
Co-polymer of crosslinked bisacrylamide and azlactone |
>4mg |
Bulk resin (2, 5mL) |
Properties of Thermo Scientific UltraLink Resin:
- Support pH Stability: 1 to 13
- Average Particle Size: 50 to 80 microns
- Exclusion Limit: greater than 2,000,000 daltons
- Average Surface Area: greater than 250m2 per gram of beads
- Average Pore Volume: greater than 1.2mL per gram of beads (>60% of bead volume)
- Pore Size: 500 to 1000 angstroms (50 to 100nm)
- Maximum Linear Velocity: 3000cm per hour
- Maximum Pressure: 100psi (6.9 bar)
References (all Streptavidin Resins):
- Nielsen K. H. et al. (2011) Synergistic activation of eIF4A by eIF4B and eIF4G. Nucleic Acids Res. 39, 2678-89.
- Havel L. S. et al. (2011) Preferential accumulation of N-terminal mutant huntingtin in the nuclei of striatal neurons is regulated by phosphorylation. Hum. Mol. Genet. 20, 1424-37.
- Caraballo J. C. et al. (2011) Hypoxia increases transepithelial electrical conductance and reduces occludin at the plasma membrane in alveolar epithelial cells via PKC-{zeta} and PP2A pathway. Am J Physiol Lung Cell Mol Physiol. 300, L569-78.
- Nielsen K. H. et al. (2009) Mechanism of ATP turnover inhibition in the EJC. RNA. 15, 67-75.
- Lamkanfi M. et al. (2008) Targeted peptidecentric proteomics reveals caspase-7 as a substrate of the caspase-1 inflammasomes. Mol. Cell. Proteomics. 7, 2350-63.
Related Products:
Other Biotin-binding Affinity Resins
Purified Streptavidin and Streptavidin Conjugates
Streptavidin Coated Plates
Pierce Streptavidin Magnetic Beads
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