Precast Tris-glycine gels with a long shelf-life and short run time.
Thermo Scientific Precise Tris-Glycine Gels are long shelf-life, fast run-time polyacrylamide gels for SDS-PAGE that use the popular Laemmli buffer system and commercial gel tanks to provide outstanding protein resolution and separation.
Precise Tris-Glycine Gels are cast in durable 10 x 8cm plastic cassettes to fit in Mini-PROTEAN* gel tanks. The gels are individually packaged in easy-to-open plastic pouches and are ready for electrophoresis with no comb or tape to remove. Following electrophoresis, the easy-open cassette design allows gels to be removed damage-free without special tools. Precise Gels in the 10 x 8cm size are available in gradient (4 to 20% and 8 to 16%) and fixed (8%, 10% and 12%) concentrations and in 10-well, 12-well and 15-well formats.
Highlights:
High-performance – Tris-glycine running buffer produces excellent separation and high-resolution protein bands
Long shelf-life – up to 12-month stability ensures consistent performance for the duration of each package of gels
Fast – 45-minute run-time provides results quickly; transfers efficiently to nitrocellulose and PVDF membranes; stains quickly with coomassie and silver stains
Compatible – these 10 x 8cm cassettes fit Bio-Rad* Mini-PROTEAN* and Hoefer* Mighty Small II gel tanks, as well as other similar tanks
Easy to load – sample wells reinforced with plastic to eliminate damage when loading and well-to-well contamination
Specifications:
Cassette Dimensions: 10cm x 8.0cm x 5mm
Gel Dimensions: 8 cm x 6.8cm x 1mm
Storage Conditions: 4°C
Shelf Life: up to 12 months from date of purchase
Stacking Gel: 4%
Buffer System in Gel: Tris-HCl, pH 7
SDS: None
Required Running Buffer: Tris-glycine-SDS
Recommended Sample Buffer: Tris-HCl-SDS or LDS-SDS
Gel Tank Compatibility (10cm x 8cm x 5mm):
Bio-Rad Mini-PROTEAN II and 3
Bio-Rad Mini-PROTEAN Tetra Cell
Hoefer Tall Mighty Small (SE 280)
Hoefer Mighty Small II (SE 260)
IBI Scientific Universal Protein Systems
For gels that are compatible with other tanks, see our 10 x 10cm Gels
Product Details:
Precise Tris-Glycine Gels were developed to work with the Laemmli Tris-glycine running buffer. Unlike other Tris-glycine SDS-PAGE gels, Precise Tris-Glycine Gels are cast in a neutral buffer system, which allows them to have a long shelf-life. Furthermore, Precise Tris-Glycine Gels can withstand the chemical changes caused by the Laemmli running-buffer that alter gel conductivity and increase run-times in other Tris-glycine gels. As a result, Precise Tris-Glycine Gels allow users to achieve excellent protein resolution in a shorter time.
Comparison of 4-20% gradient Tris-glycine gels. The same set of protein samples (MW markers, pure proteins, cell lysates) were separated and then stained on a 4-20% Thermo Scientific Precise Tris-Glycine Gel and a 4-20% Mini-PROTEAN TGX Precast Gel. The Precise Gel provided band resolution over a greater portion of the gel height.
