Microplate kits for sandwich ELISA of human vascular endothelial growth factor (VEGF).
Assay VEGF-A (human vascular endothelial growth factor) levels in human serum, plasma (heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Human VEGF ELISA Kits.
These human VEGF-A ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of vascular endothelial growth factor (VEGF-A) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Target: natural and recombinant human VEGF-A (vascular endothelial growth factor); see Product Details for additional information
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Specificity: no significant cross-reaction with human VEGF ELISA: human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12, IL-12p40, IL-13, IL-15, IL-16, IL-17, PLGF-1, PLGF-2, RANTES, TGFβ, TNFβ, VEGF-B, VEGF-C or VEGF-D; rat VEGF; mouse VEGF120 or VEGF164.
Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a "MiniKit") contains only matched antibodies and standard (no plates or detection reagents).
Human VEGF ELISA standard curve. An example standard curve for the Thermo Scientific Human VEGF ELISA Kit (Part No. EH2VEGF), with error bars based on six replicates for each VEGF standard concentration. Also see standard curves for the VEGF-A Reagent Kit and VEGF-A Antibody Pair Kit.