Pierce 96-well Microdialysis Plates

The Thermo Scientific Pierce 96-well Microdialysis Plates are automation-compatible, microplate dialysis devices (3.5K or 10K MWCO) for rapidly processing 1 to 96 samples with volumes 10 to 100µL.

Each sample processed with the Pierce 96-well Microdialysis Dialysis Plate is dialyzed inside a single, removable dialysis device created by two regenerated cellulose membranes separated by ~2mm. The short diffusion distance and large surface area facilitates rapid dialysis with the removal of salts and small molecules often completed in 2 to 4 hours. Sample recovery is performed using standard laboratory micropipettes. Individual microdialysis devices can also be used with 2mL microcentrifuge tubes for processing single samples. The available plate seals are made of high-quality polypropylene and help protect samples from contamination and evaporation, especially during long-term storage.

Highlights:

• High yield – greater than 90% protein recovery after dialysis
• Rapid – dialysis completed in 2 to 4 hours
• Easy to use – sample loading and retrieval can be done with a standard pipette
• Flexible – detachable 8-device strips, scalable from 1 to 96 samples
• Automation-compatible – plate format conforms to the SBS Microplate Standard

Applications:

• Protein and DNA desalting/buffer exchange
• Removal of dyes after protein labeling
• Enzyme activity/binding assays
• Drug binding studies (also see Rapid Equilbrium Dialysis "RED" Devices)

Product Details:

Each 96-well Microdialysis Plate comes pre-loaded with twelve 8-dialysis device strips. Each dialysis device strip can be removed from the plate and individual microdialysis devices easily separated, allowing only the number of needed devices to be used and eliminating waste. The sample chamber inside each microdialysis device is formed by two low-binding regenerated cellulose membranes that are rated to retain proteins and other macromolecules >10,000 Daltons while allowing removal of buffer salts and contaminants <1000 Daltons. Surface tension and the small distance between the membranes facilitate highly efficient sample recovery using standard laboratory pipettes.

Protocol summary for Thermo Scientific 96-well MicroDialysis Plate.

Using the Microdialysis Plate, sample dialysis can be efficiently performed in a standard 96 deep-well plate with a minimal amount of buffer while still providing >95% removal of small molecules. The assembled device is compatible with standard 96-well laboratory equipment and automated liquid-handling systems making it an ideal option for high-throughput applications. Single microdialysis devices can be used independently in 2mL microcentrifuge tube for processing single samples.

Rate of removal of NaCl in the Thermo Scientific Pierce 96-well Microdialysis Device, 10K MWCO. Samples of 0.1mL (0.4mg/mL cytochrome c containing 1M NaCl) were dialyzed against 1.8mL of water in a 96 deep-well plate at RT with gentle shaking on a plate shaker. The buffer was changed at 1-, 2- and 3-hour intervals over a 4-hour period. The rate of NaCl removal was determined by measuring the conductivity of the retentate at the indicated time intervals. Removal of NaCl was >83% after 2 hours and >99% after 4 hours.

Related Resources:

Review of Dialysis Methods
Dialysis Products Selection Guide

Related Products:

Slide-A-Lyzer MINI Dialysis Devices (volumes 10 to 2000µL)
Rapid Equilibrium Dialysis Devices (volumes 100 to 500µL)
Slide-A-Lyzer Dialysis Concentrating Solution
Dialysis Tubing, Cassettes and Systems
Desalting Columns
Pierce Protein Concentrators
Buffers for Protein Methods