High-performance fluorescent probes for biotinylated antibody detection.
Thermo Scientific Pierce Streptavidin DyLight Conjugates are preparations of streptavidin biotin-binding protein that are tagged with Thermo Scientific DyLight Fluors for use in high-performance fluorescence assays and cellular imaging.
The streptavidin biotin-binding protein is offered as highly functional conjugates with each of nine different DyLight Fluorescent Dyes having absorption spectra ranging from 350nm to 777nm and bright emission spectra that match the principal output wavelengths of common fluorescence microscopes and imagers. DyLight Conjugates exhibit higher fluorescence intensity and photostability than Alexa Fluor*, Cy* and LI-COR* Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9).
Highlights:
Streptavidin protein – choose the biotin-binding protein that is best for your application
Optimized conjugates – manufactured with optimal dye:protein ratios to provide the highest possible binding function and overall fluorescence intensity
Consistent and stable – all conjugates are stable for 1 year at 4°C, providing consistent performance for the duration of use
DyLight Fluorophore Highlights:
Bright fluorescence – intense emission provides superior sensitivity and requires less conjugate
Excellent photostability – exceptional resistance to photobleaching enables fluorescence imaging under the most demanding conditions (e.g., structured illumination, 4Pi microscopy)
Buffer stability – conjugated fluorophores are completely stable at pH 4-9
Instrument compatibility – excitation and emission spectra correspond with filter sets and laser settings of all popular fluorescence instrumentation
Emission spectra of Thermo Scientific DyLight Fluors 350-800 for which Pierce Streptavidin Conjugates are available. Line colors represent the approximate visible colors of the wavelength maxima.
