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Sulfhydryl-to-Sulfhydryl Crosslinkers


Sulfhydryl-specific crosslinking reagents based on maleimide or pyridyldithiol reactive groups for selective covalent conjugation of protein and peptide thiols (reduced cysteines) to form stable thioether bonds.


Long, pegylated, sulfhydryl-to-sulfhydryl crosslinker; composed of maleimide groups and 2-or 3-unit polyethylene glycol spacer arm; water-soluble. 

Mid-length (10.9A), sulfhydryl-to-sulfhydryl crosslinker; composed of maleimide groups and 9-atom spacer arm; conjugate protein or peptide cysteines. 

Mid-length (10.2A), periodate-cleavable, sulfhydryl-to-sulfhydryl crosslinker; composed of maleimide groups and 9-atom spacer arm; conjugate protein or peptide cysteines. 

Long (13.0A), sulfhydryl-to-sulfhydryl crosslinker; composed of maleimide groups and 11-atom spacer arm; conjugate protein or peptide cysteines. 

Short (8.0A), sulfhydryl-to-sulfhydryl crosslinker; composed of maleimide groups and 7-atom spacer arm; conjugate protein or peptide cysteines. 

Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker; composed of maleimide groups and 11-atom disulfide spacer arm; conjugate protein or peptide cysteines. 

A 3-way, sulfhydryl-to-sulfhydryl crosslinker; tris (2-maleimidoethyl) amine; composed of three maleimide groups on short spacer arms; conjugate protein or peptide cysteines. 

 

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