This equine TNF alpha ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of horse tumor necrosis factor alpha (TNFα) in samples at 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.
Format: colorimetric ELISA (TMB substrate) for 96-well microplates
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 16 to 1000pg/mL
Sensitivity: undetermined
Sample Types: cell culture supernatant
Total Assay Time: 3 hours
Sample Size: 100μL
Specificity: no significant cross-reaction with bovine, mouse, or rat TNFα. Partial cross-reaction occurs with human and porcine TNFα (< 10%).
Includes:
The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.
Details:
Equine TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Equine TNF alpha ELISA Reagent Kit (Part No. ESS0017), with error bars based on six replicates for each tumor necrosis factor alpha standard concentration.
