Amine-reactive azide labeling reagents for azide-phosphine chemoselective ligation.
Thermo Scientific NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.
These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different phosphine-activated compounds, these NHS-Azide reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.
Highlights:
Soluble – reagents easily dissolve in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency
Pegylated – choose the very short NHS-Azide or one of the two varieties whose spacer arms contain medium (4-unit) or long (12-unit) polyethylene glycol (PEG) groups for increased solubility and reach
Product Details:
Chemical structures of NHS-Azide Reagents. These reagents label proteins and molecules that contain primary amines (e.g., side-chain of lysine) via the NHS Ester Reaction Chemistry. Labeled molecules will contain an azide group (N3), which can be conjugated and detected using phosphine-activated probes via the Staudinger Ligation Reaction Chemistry.
Properties of NHS-Azide reagents. These compounds are identical except for the their overall length as a result of different numbers of polyethylene glycol groups (PEG units).
Formulation: Tetraethyleneglycol functionalized with succinimidyl and azido ends; liquid Sufficient For: 257mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
100mg
$236.00
26131
NHS-PEG12-Azide
Formulation: 12-unit ethyleneglycol functionalized with succinimidyl and azido ends; liquid Sufficient For: 135mL of typical 1X (1mM) reaction solution, or approx. 50 uses at 2mg reagent per use
100mg
$262.00
88902
NHS-Azide
Formulation: Ethane functionalized with succinimidyl and azido ends; solid Sufficient For: 50mL of typical 1X (1mM) reaction solution, or approx. 5 uses at 2mg reagent per use
