Thermo Scientific Pierce Phosphine-PEG3-Biotin is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.
When used in combination with azide labeling strategies, this compound enables detection or affinity purification of protein interactions and post-translational modifications using streptavidin probes or streptavidin agarose resins. The phosphine group of Phosphine-PEG3-Biotin conjugates to azide groups by the Staudinger reaction mechanism. Azide groups can be introduced into proteins or other cellular targets through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks (bioorthogonal compounds). Because neither phosphines nor azides are present in biological systems, they comprise a chemoselective (mutually specific) ligation pair for labeling and conjugation.
Highlights:
- Soluble – easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
- Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
- Chemoselective – the phosphine reactive group is specific in biological samples for azide-tagged molecules, ensuring that biotinylation is specific
- PEG spacer – polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein
Product Details:
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Properties of Phosphine-PEG3-Biotin.
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| Alternative names |
Phosphine-PEO3-Biotin, Phosphine-dPEG3-Biotin |
| Molecular formula |
C41H53N4O8PS |
| SMILES structure |
O=C(NCCCOCCOCCOCCCNC(C1=CC(P(C2=CC=CC=C2)C3=CC=CC=C3)=C(C(OC)=O)C=C1)=O) CCCCC(SC4)C5C4NC(N5)=O (NOTE: remove character space between rows of text string) |
| Molecular weight |
792.92 |
| Spacer arm length |
33.0 angstroms |
| Form |
Light yellow to yellow powder |
| Storage conditions |
-20°C |
| Reactive groups |
Phosphine, reacts with azide groups |
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| Example strategy for in vivo metabolic labeling with biotin using Phosphine-PEG3-Biotin. When bioorthogonal azido-sugar derivatives are supplied to living cells, they become incorporated into glycoprotein structures by endogenous post-translational modification mechanisms. The azide-tagged molecules can then be selectively labeled or conjugated to phosphine-activated molecules, in this case, a derivative of biotin. If the azido sugars were supplied to cells being studied for response to a particular treatment, the biotin affinity-tag could be used to purify and analyze differences in glycosylation resulting from the treatment regime. |
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