Thermo Scientific Pierce Hydrochloric Acid Solution is a constant-boiling 6N HCl solution, supplied in 1mL ampules, that is specifically formulated as a sequencing-grade reagent for total protein hydrolysis for amino acid analysis.
A variety of products are available for peptide hydrolysis, amino acid analysis and HPLC separation. This constant boiling HCl is specially purified to effect peptide hydrolysis at 150°C, to ensure the integrity of your amino acid analysis data and to maximize sensitivity by eliminating interferences in your amino acid analysis. Pierce Hydrochloric Acid is purified and packaged to ensure a ninhydrin negative blank on hydrolysis. Convenient, pre-scored ampule packaging of the ready-to-use HCl maintains reagent integrity. This virtually eliminates exposure to laboratory atmospheres, fingerprints and other contaminants resulting from pipetting from bulk bottles.
Highlights:
- Hydrolyzes peptides in 6 hours at 150°C
- Specially purified to give a ninhydrin-negative blank on hydrolysis
- Packaged in ampules to eliminate contamination and ensure product integrity
Product Details:
Eveleigh and Winter (1970) give an excellent description of the total protein hydrolysis technique using Constant Boiling Hydrochloric Acid. Standard protein hydrolysis conditions are 105-110°C for 16-24 hours. At 150°C, this reagent can hydrolyze peptides in 6 hours.
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Chemical structure of hydrochloric acid (HCl). |
References:
- Eveleigh, J.W. and Winter, G.D. (1970). Protein Sequence Determination, Ed Needleman, S.B., Springer-Verlag, pp 92-95.
- Blankenship, et al. (1989). High-sensitivity amino acid analysis by derivatization with o-Phthalaldehyde and 9-Fluorescence detection: applications in protein structure determination. Anal. Biochem. 178, 227-232.
- HurIey, J.B., et al. (1984). Isolation and characterization of a cDNA clone for the λ subunit of bovine retinal transducin. Proc. Natl. Acad. Sci. U.S.A. 81, 6948-6952.
- Lee, K., et al. (1979). Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-Phthaldialdehyde/ 2-mercaptoethanol reagent. J. Biol. Chem. July 25, 6248-6251.
Related Products:
Vacuum Hydrolysis Tubes
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Amino Acid Detection Reagents
Solvents for HPLC
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