Thermo Scientific PageRuler Unstained Low Range Protein Ladder is a mixture of seven (7) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small-protein gel electrophoresis.
The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag* II Sequence and may be detected on Western blots using Strep-Tactin* Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.
- Size range – seven proteins and one peptide spanning 3.4 to 100kDa
- Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
- Sharp bands – excellent accuracy for detection in with coomassie or silver stains
- Reference bands – 25kDa band is stronger than the others for easy orientation
- Tagged – each protein in the ladder contains an integral Strep-tag* II Sequence
- Quality tested – each lot evaluated by SDS-PAGE and Western blotting
- Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol
- Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
SDS-PAGE band profile of the Thermo Scientific PageRuler Unstained Low Range Protein Ladder. Image is of an 18% Tris-tricine small-peptide SDS-polyacrylamide gel stained with coomassie blue dye.
- Shägger, H., and von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to100 kDa, Anal. Biochem, 166, 368-379.
- Westermeier, R. (2004). Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations, 4th Edition, Wiley.
- Kurien, B.T. and Scofield, R.H. (2003). Protein blotting: a review, J. Imm. Meth., 274, 1-15.
Protein Molecular Weight Markers – product comparison and selection guide
Protein Gel Stains and Kits – compare colorimetric and fluorescent gel stains
Precise Protein Gels – browse all pre-cast gels for SDS-PAGE
BupH Dry Blend Electrophoresis Buffers – Tris-HEPES-SDS and other pouched electrophoresis buffers
Reducing Agents – 2-mercaptoethanol, DTT, TCEP and other reducing agents
Western blotting substrates and reagents – find kits, stripping buffers, blotting membranes and film