Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.
This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.
- Size range – nine proteins spanning 10 to 250kDa
- Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil
- Sharp bands – color-coded bands of similar intesity for easy visualization
- Quality tested – each lot evaluated by SDS-PAGE and Western blotting
- Bright reference bands – orange at 70 and 25kDa, and green at 10kDa
- Membrane-compatible – colored bands transfer to membranes for Western blotting
- Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
- Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
- Monitoring protein transfer onto membranes after Western blotting
- Sizing of proteins on SDS-polyacrylamide gels and Western blots
SDS-PAGE band profile of the Thermo Scientific PageRuler Plus Prestained Protein Ladder. Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane.
Migration patterns of PageRuler Plus Prestained Protein Ladder in different electrophoretic conditions. The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.
- Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685.
- Burnette, W.N. (1981). Western blotting: electrophoretic transfer of proteins from sodium dodecyl sulfate – polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A, Anal. Biochem., 112 (2), 195-203.
- Towbin, H., et al. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA, 76, 4350-4354.
Protein Molecular Weight Markers – product comparison and selection guide
Protein Gel Stains and Kits – compare colorimetric and fluorescent gel stains
Precise Protein Gels – browse all pre-cast gels for SDS-PAGE
BupH Dry Blend Electrophoresis Buffers – Tris-HEPES-SDS and other pouched electrophoresis buffers
Reducing Agents – 2-mercaptoethanol, DTT, TCEP and other reducing agents
Western blotting substrates and reagents – find kits, stripping buffers, blotting membranes and film