Thermo Scientific Unstained Protein Molecular Weight Marker is a mixture of seven (7) native proteins (14.4kDa to 116kDa) for use as size standards in protein electrophoresis (SDS-PAGE).
This protein MW marker is designed for accurate sizing of proteins in SDS-polyacrylamide gel electrophoresis, as well as on PVDF, nylon and nitrocellulose membranes. The protein standard produces sharp bands on protein mini gels following staining with Thermo Scientific Pierce Gel Stains or other coomassie dye or silver stains.
- Size range – seven proteins spanning 14.4 to 116kDa
- Ready-to-use – supplied in a loading buffer for direct loading on gels
- Sharp bands – equal intensity upon coomassie or silver staining
- Quality tested – each lot evaluated by SDS-PAGE and Western blotting
- Proteins (0.1-0.2mg/mL of each) in 62.5mM Tris-HCl (pH 7.0 at 25°C), 1mM EDTA, 2% SDS, 50mM DTT, 30mM NaCl, 1mM NaN3, 0.01% bromophenol blue and 50% glycerol
- Component proteins: beta-galactosidase (116kDa), bovine serum albumin (66.2kDa), ovalbumin (45.0kDa), lactate dehydrogenase (35.0kDa), REase Bsp98I (25.0kDa), beta-lactoglobulin (18.4kDa), lysozyme (14.4kDa)
- Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
SDS-PAGE band profile of the Thermo Scientific Unstained Protein Molecular Weight Marker. Image is a 12% Tris-glycine gel (SDS-PAGE) stained with coomassie blue gel stain.
- Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-685.
- Fowler, A.V., Zabin, I. (1977). The amino acid sequence of beta-galactosidase of Escherichia coli, Proc. Natl. Acad. Sci. USA, 74, 1507-1510.
- Brown, J.R. (1975). Structure of bovine serum albumin, Fed. Proc., 34, 591.
- Warner, R.C. (1954). Egg proteins, Proteins, II A., 435, (Neurath, H., Bailey, K., eds.), Academic Press, NY.
- Castellino, F.J., Barker, R. (1968). Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometry, Biochemistry, 7, 2207-2217.
- Unpublished results.
- Dayhoff, M. (1969). Atlas of Protein Sequence and Structure, vol.4, National Biomedical Research Foundation, Silver Spring, MD.
- Jolles, P. (1969). Lysozymes: A chapter of molecular biology, Angew. Chem., Intl. Edit., 8, 227-294.
Protein Molecular Weight Markers – product comparison and selection guide
Protein Gel Stains and Kits – compare colorimetric and fluorescent gel stains
Precise Protein Gels – browse all pre-cast gels for SDS-PAGE
BupH Dry Blend Electrophoresis Buffers – Tris-HEPES-SDS and other pouched electrophoresis buffers
Reducing Agents – 2-mercaptoethanol, DTT, TCEP and other reducing agents
Western blotting substrates and reagents – find kits, stripping buffers, blotting membranes and film